The presence of Nipah virus in respiratory secretions and urine of patients during an outbreak of Nipah virus encephalitis in Malaysia

Chua, K.B.; Lam, S.K.; Goh, K.J.; Hooi, P.S.; Ksiazek, T.G.; Kamarulzaman, A.; Olson, J.; Tan, C.T. (2001) The presence of Nipah virus in respiratory secretions and urine of patients during an outbreak of Nipah virus encephalitis in Malaysia. Journal of Infection, 42 (1). pp. 40-43. ISSN 0163-4453

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    Abstract

    Objectives: To study the excretion of Nipah virus in the upper respiratory secretions and urine of infected patients in relation to other clinical features. Methods: Isolation of Nipah virus from the respiratory secretions and urine was made in Vero cells and identified by indirect immunofluorescence assay using anti-Hendra specific hyperimmune mouse ascitic fluid and FITC-conjugated goat anti-mouse IgG. Results: During the peak outbreak of Nipah virus encephalitis in Malaysia, Nipah virus was isolated from the upper respiratory secretions and urine in eight of 20 patients who were virologically and/or serologically confirmed to be infected with the virus, From these eight patients, Nipah virus was isolated from sire throat swab specimens, three urine specimens and only one nasal swab specimen. The positive virus isolation rate was related to the collection of these specimens during the early phase of the illness (P = 0.068). The presence of serum anti-Nipah specific IgM appeared to reduce the chance of isolating the virus (P = 0.049). There was no significant difference in the isolation rate with respect to the age, gender, ethnic group and clinical features associated with grave prognosis and mortality outcome of the patients. Conclusion: This study shows that it is possible to be infected from secretions of infected patients, but epidemiological survey on close contacts so far did not suggest that human-to-human transmission is common. (C) 2001 The British Infection Society.

    Item Type: Article
    Creators:
    1. Chua, K.B.
    2. Lam, S.K.
    3. Goh, K.J.
    4. Hooi, P.S.
    5. Ksiazek, T.G.
    6. Kamarulzaman, A.(Department of Medicine, Faculty of Medicine Building, University of Malaya, 50603 Kuala Lumpur, MALAYSIA)
    7. Olson, J.
    8. Tan, C.T.
    Journal or Publication Title: Journal of Infection
    Additional Information: Chua, KB Lam, SK Goh, KJ Hooi, PS Ksiazek, TG Kamarulzaman, A Olson, J Tan, CT
    Uncontrolled Keywords: Nipah virus
    Subjects: R Medicine
    Divisions: Faculty of Medicine
    Depositing User: Ms azrahani halim
    Date Deposited: 04 Dec 2012 11:03
    Last Modified: 04 Dec 2012 11:04
    URI: http://eprints.um.edu.my/id/eprint/4607

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