Jabbar, Ahmed A. J. and Alamri, Zaenah Zuhair and Abdulla, Mahmood Ameen and Salehen, Nur Ain and Ibrahim, Ibrahim Abdel Aziz and Hassan, Rawaz Rizgar and Almaimani, Ghassan and Bamagous, Ghazi A. and Almaimani, Riyad A. and Almasmoum, Hussain A. and Ghaith, Mazen M. and Farrash, Wesam F. and Almutawif, Yahya A. (2024) Boric Acid (Boron) attenuates AOM-induced colorectal cancer in rats by augmentation of apoptotic and antioxidant mechanisms. Biological Trace Element Research, 202 (6). pp. 2702-2719. ISSN 0163-4984, DOI https://doi.org/10.1007/s12011-023-03864-0.
Full text not available from this repository.Abstract
Boric acid (BA) is a naturally occurring weak Lewis acid containing boron, oxygen, and hydrogen elements that can be found in water, soil, and plants. Because of its numerous biological potentials including anti-proliferation actions, the present investigates the chemopreventive possessions of BA on azoxymethane (AOM)-induced colonic aberrant crypt foci (ACF) in rats. Thirty laboratory rats were divided into 5 groups: negative control (A) received two subcutaneous inoculations of normal saline and nourished on 10% Tween 20; groups B-E had two injections of 15 mg/kg azoxymethane followed by ingestion of 10% Tween 20 (B, cancer control), inoculation with intraperitoneal 35 mg/kg 5-fluorouracil injection (C, reference group), or ingested with boric acid 30 mg/kg (D) and 60 mg/kg (E). The gross morphology results showed significantly increased total colonic ACF in cancer controls, while BA treatment caused a significant reduction of ACF values. Histopathological evaluation of colons from cancer controls showed bizarrely elongated nuclei, stratified cells, and higher depletion of the submucosal glands than that of BA-treated groups. Boric acid treatment up-surged the pro-apoptotic (Bax) expression and reduced anti-apoptotic (Bcl-2) protein expressions. Moreover, BA ingestion caused upregulation of antioxidant enzymes (GPx, SOD, CAT), and lowered MDA contents in colon tissue homogenates. Boric acid-treated rats had significantly lower pro-inflammatory cytokines (TNF-alpha and IL-6) and higher anti-inflammatory cytokines (IL-10) based on serum analysis. The colorectal cancer attenuation by BA is shown by the reduced ACF numbers, anticipated by its regulatory potentials on the apoptotic proteins, antioxidants, and inflammatory cytokines originating from AOM-induced oxidative damage.
| Item Type: | Article |
|---|---|
| Funders: | UNSPECIFIED |
| Uncontrolled Keywords: | Colon cancer; ACF; Boric acid; Cytokines; Histology; Immunohistochemistry |
| Subjects: | R Medicine |
| Divisions: | Faculty of Medicine > Biomedical Science Department |
| Depositing User: | Ms. Juhaida Abd Rahim |
| Date Deposited: | 06 Nov 2024 05:43 |
| Last Modified: | 06 Nov 2024 05:43 |
| URI: | http://eprints.um.edu.my/id/eprint/46008 |
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