Jabbar, Ahmed A. J. and Ahmed, Khaled Abdul-Aziz and Abdulla, Mahmood Ameen and Abdullah, Fuad Othman and Salehen, Nur Ain and Mothana, Ramzi A. and Houssaini, Jamal and Hassan, Rawaz Rizgar and Hawwal, Mohammed F. and Fantoukh, Omer I. and Hasson, Sidgi (2024) Sinomenine accelerate wound healing in rats by augmentation of antioxidant, anti-inflammatory, immunuhistochemical pathways. Heliyon, 10 (1). e23581. ISSN 2405-8440, DOI https://doi.org/10.1016/j.heliyon.2023.e23581.
Full text not available from this repository.Abstract
Sinomenine (SN) is a well-documented unique plant alkaloid extracted from many herbal med-icines. The present study evaluates the wound healing potentials of SN on dorsal neck injury in rats. A uniform cut was created on Sprague Dawley rats (24) which were arbitrarily aligned into 4 groups receiving two daily topical treatments for 14 days as follows: A, rats had gum acacia; B, rats addressed with intrasite gel; C and D, rats had 30 and 60 mg/ml of SN, respectively. The acute toxicity trial revealed the absence of any toxic signs in rats after two weeks of ingestion of 30 and 300 mg/kg of SN. SN-treated rats showed smaller wound areas and higher wound closure per-centages compared to vehicle rats after 5, 10, and 15 days of skin excision. Histological evaluation of recovered wound tissues showed increased collagen deposition, fibroblast content, and decreased inflammatory cells in granulated tissues in SN-addressed rats, which were statistically different from that of gum acacia-treated rats. SN treatment caused positive augmentation of Transforming Growth Factor Beta 1 (angiogenetic factor) in wound tissues, denoting a higher conversion rate of fibroblast into myofibroblast (angiogenesis) that results in faster wound healing action. Increased antioxidant enzymes (SOD and CAT), as well as decreased MDA con-tents in recovered wound tissues of SN-treated rats, suggest the antioxidant potentials of SN that aid in faster wound recovery. Wound tissue homogenates showed higher hydroxyproline amino acid (collagen content) values in SN-treated rats than in vehicle rats. SN treatment suppressed the production of pro-inflammatory cytokines and increased anti-inflammatory cytokines in the serum of wounded rats. The outcomes present SN as a viable pharmaceutical agent for wound healing evidenced by its positive modulation of the antioxidant, immunohistochemically proteins, hydroxyproline, and anti-inflammatory cytokines.
| Item Type: | Article |
|---|---|
| Funders: | King Saud University (RSP2023R119) |
| Uncontrolled Keywords: | Sinomenine; Wound; Histology; Antioxidants; Immunohistochemicals; Inflammatory cytokines |
| Subjects: | R Medicine > R Medicine (General) |
| Divisions: | Faculty of Medicine > Biomedical Science Department |
| Depositing User: | Ms. Juhaida Abd Rahim |
| Date Deposited: | 15 Nov 2024 01:07 |
| Last Modified: | 15 Nov 2024 01:07 |
| URI: | http://eprints.um.edu.my/id/eprint/45950 |
Actions (login required)
 |
View Item |
