Chen, Chow Jie and Lokesh, Bontha Venkata Subrahmanya and Akowuah, Gabriel Akyirem (2024) Quantitative Applications of ATR-FTIR Spectroscopy with Chemometrics for the Estimation of Amikacin in Amikacin Sulphate Injections. Current Analytical Chemistry, 20 (3). pp. 201-208. ISSN 1573-4110, DOI https://doi.org/10.2174/0115734110278516240129174949.
Full text not available from this repository.Abstract
Background Amikacin belongs to the class of aminoglycoside antibiotics used in the treatment of gram-negative bacterial infections. It is resistant to the aminoglycosides modifying enzymes, making it a clinically effective drug in multidrug-resistant infections.Methods In this study, a simple Attenuated Total Reflectance-Fourier Transform Infrared Spectroscopy was used for the quantification of amikacin in amikacin sulphate injection. The infrared spectra were generated in the spectral range of 4000-667 cm-1. The calibration curve was computed through TQ Analyst Pro edition software, and the partial least square regression analysis found the linearity in the range of 10-60% w/w.Results The best calibration results were obtained in the spectral region from 1040 to 1020 cm-1 with a correlation coefficient (r2) of 1.000. The residual mean standard error (RMSEC) value was 0.00235. The percent relative standard deviation (%RSD) values for intra-day and inter-day precision were less than 8.0. The percent relative error (%RE) values were calculated and found in between the range of 0.52 to 5.60. The percent recovery of the amikacin estimation was 113.09 +/- 4.27(n=3).Conclusion This validated method is considered a green method, which is suitable for the routine analysis of amikacin in amikacin sulphate injections.
| Item Type: | Article |
|---|---|
| Funders: | UCSI University Research Grant Scheme - Centre of Excellence for Research, Innovation, and Entrepreneurship (CERVIE), UCSI University, Kuala Lumpur, Malaysia (Proj-In-FPS-016) |
| Uncontrolled Keywords: | Aminoglycoside; injections; attenuated total reflectance; fourier transform infrared spectroscopy; PLS; amikacin |
| Subjects: | R Medicine > RM Therapeutics. Pharmacology R Medicine > RS Pharmacy and materia medica |
| Divisions: | Faculty of Pharmacy > Department of Pharmaceutical Chemistry |
| Depositing User: | Ms. Juhaida Abd Rahim |
| Date Deposited: | 13 Nov 2024 03:57 |
| Last Modified: | 13 Nov 2024 03:57 |
| URI: | http://eprints.um.edu.my/id/eprint/45846 |
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