Fluoroquinolone resistance in clinical isolates of Streptococcus pneumoniae from Asian countries: ANSORP study

Oh, W.S. and Suh, J.Y. and Song, J.H. and Ko, K.S. and Jung, S.I. and Peck, K.R. and Lee, N.Y. and Yang, Y.H. and Chongthaleong, A. and Chiu, C.H. and Kamarulzaman, A. and Parasakthi, N. and Lalitha, M.K. and Perera, J. and Yee, T.T. and Kumarasinghe, G. and Carlos, C.C. (2004) Fluoroquinolone resistance in clinical isolates of Streptococcus pneumoniae from Asian countries: ANSORP study. Microbial Drug Resistance, 10 (1). pp. 37-42. ISSN 1076-6294

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Abstract

Seventeen clinical isolates of Streptococcus pneumoniae showing reduced susceptibility to ciprofloxacin (MIC : 4 mug/ml) collected from eight different Asian countries were analyzed by antimicrobial susceptibility, serotyping, pulsed-field gel electrophoresis (PFGE), and DNA sequencing of the quinolone resistance-determining regions (QRDRs) in gyrA, gyrB, parC, and parE. All isolates but one showed more than one amino acid alteration in QRDRs of four responsible genes. Ile460 —> Val in parE was the most common mutation. Data suggest that Lys137 —> Asn in parC may be a primary step in the development of high-level and multiple FQ resistance. An additional mutation of Ser81 —> Phe in gyrA resulted in high-level resistance to ciprofloxacin, levofloxacin, and gatifloxacin, whereas Ser79 —> Phe in parC may exert an important role in the development of moxifloxacin resistance. Two novel amino acid changes in gyrB, Ala390 —> Val and Asn423 —> Thr, were found. Data from PFGE suggest an introduction and local spread of multiple resistant Spain(23)F-1 clone in Hong Kong, but isolates from other Asian countries were not related to this clone.

Item Type: Article
Additional Information: Oh, WS Suh, JY Song, JH Ko, KS Jung, SI Peck, KR Lee, NY Yang, YH Chongthaleong, A Chiu, CH Kamarulzaman, A Parasakthi, N Lalitha, MK Perera, J Yee, TT Kumarasinghe, G Carlos, CC
Uncontrolled Keywords: Fluoroquinolone Resistance
Subjects: R Medicine
Divisions: Faculty of Medicine
Depositing User: Ms azrahani halim
Date Deposited: 23 Jan 2013 08:11
Last Modified: 15 Jul 2019 04:01
URI: http://eprints.um.edu.my/id/eprint/4582

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