Lau, Katherine A. and Wang, Bin and Miranda-Saksena, Monica and Boadle, Ross and Kamarulzaman, Adeeba and Ng, Kee Peng and Saksena, Nitin K. (2010) Evidence for Possible Biological Advantages of the Newly Emerging HIV-1 Circulating Recombinant Form from Malaysia - CRF33_01B in Comparison to its Progenitors _ CRF01_AE and Subtype B. Current HIV Research, 8 (3). pp. 259-271. ISSN 1570-162X, DOI https://doi.org/10.2174/157016210791111151.
Full text not available from this repository.Abstract
In Malaysia, co-circulation of CRF01_AE and subtype B has resulted in the emergence of the second generation derivative; CRF33_01B in approximately 20% of its HIV-1 infected individuals. Our objective was to identify possible biological advantages that CRF33_01B possesses over its progenitors. Biological and molecular comparisons of CRF33_01B against its parental subtypes clearly show that CRF33_01B replicated better in activated whole peripheral blood mononuclear cells (PBMCs) and CD4+ T-lymphocytes, but not monocyte-derived macrophages (MDMs). Also, its acquired fitness was greater than CRF01_AE but not subtype B. Moreover, CRF33_01B has higher rate of apoptotic cell death and syncytia induction compared to subtype B. These adaptive and survival abilities could have been acquired by CRF33_01B due to the incorporation of subtype B fragments into the gag-RT region of its full-length genome. Our studies confirm the previously held belief that HIV-1 strains may harbor enhanced biological fitness upon recombination. We therefore estimate a possible gradual replacement of the current predominance of CRF01_AE, as well as wider dissemination of CRF33_01B, together with the identification of other new CRF01_AE/B inter-subtype recombinants in Malaysia.
| Item Type: | Article |
|---|---|
| Funders: | UNSPECIFIED |
| Additional Information: | Lau, Katherine A. Wang, Bin Miranda-Saksena, Monica Boadle, Ross Kamarulzaman, Adeeba Ng, Kee-Peng Saksena, Nitin K. |
| Uncontrolled Keywords: | HIV-1 CRF01AE; HIV-1 subtype B; HIV-1 CRF3301B; inter-subtype recombinant; biological fitness; Malaysia |
| Subjects: | R Medicine |
| Divisions: | Faculty of Medicine |
| Depositing User: | Ms azrahani halim |
| Date Deposited: | 23 Jan 2013 07:38 |
| Last Modified: | 11 Nov 2019 05:11 |
| URI: | http://eprints.um.edu.my/id/eprint/4568 |
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