Chong, Eric Tzyy Jiann and Neoh, Joveen Wan Fen and Lau, Tiek Ying and Chua, Kek Heng and Lim, Yvonne Ai-Lian and Lee, Ping-Chin (2024) Genetic diversity of the S-type small subunit ribosomal RNA gene of Plasmodium knowlesi isolates from Sabah, Malaysian Borneo and Peninsular Malaysia. Asian Pacific Journal of Tropical Medicine, 17 (2). pp. 84-90. ISSN 1995-7645, DOI https://doi.org/10.4103/apjtm.apjtm_540_23.
Full text not available from this repository.Abstract
Objective: To determine the genetic diversity of Plasmodium (P.) knowlesi isolates from Sabah, Malaysian Borneo and Peninsular Malaysia, targeting the S-type SSU rRNA gene and including aspects of natural selection and haplotype. Methods: Thirty-nine blood samples infected with P. knowlesi were collected in Sabah, Malaysian Borneo and Peninsular Malaysia. The S-type SSU rRNA gene was amplified using polymerase chain reaction, cloned into a vector, and sequenced. The natural selection and haplotype of the S-type SSU rRNA gene sequences were determined using DnaSP v6 and illustrated using NETWORK v10. This study's 39 S-type SSU rRNA sequences and eight sequences from the Genbank database were subjected to phylogenetic analysis using MEGA 11. Results: Overall, the phylogenetic analysis showed no evidence of a geographical cluster of P. knowlesi isolates from different areas in Malaysia based on the S-type SSU rRNA gene sequences. The S-type SSU rRNA gene sequences were relatively conserved and with a purifying effect. Haplotype sharing of the S-type SSU rRNA gene was observed between the P. knowlesi isolates in Sabah, Malaysian Borneo, but not between Sabah, Malaysian Borneo and Peninsular Malaysia. Conclusions: This study suggests that the S-type SSU rRNA gene of P. knowlesi isolates in Sabah, Malaysian Borneo, and Peninsular Malaysia has fewer polymorphic sites, representing the conservation of the gene. These features make the S-type SSU rRNA gene suitable for comparative studies, such as determining the evolutionary relationships and common ancestry among P. knowlesi species.
| Item Type: | Article |
|---|---|
| Funders: | Ministry of Education, Malaysia (FRGS0322-SG-1/2013), Universiti Malaysia Sabah (GUG0521-2/2020) |
| Uncontrolled Keywords: | Plasmodium knowlesi; S-type small subunit ribosomal RNA; Genetic diversity; Natural selection; Haplotype |
| Subjects: | R Medicine > RA Public aspects of medicine R Medicine > RA Public aspects of medicine > RA0421 Public health. Hygiene. Preventive Medicine |
| Divisions: | Faculty of Medicine > Biomedical Science Department Centre of Excellence for Research in AIDS (CERiA) |
| Depositing User: | Ms. Juhaida Abd Rahim |
| Date Deposited: | 07 Nov 2024 07:00 |
| Last Modified: | 07 Nov 2024 07:00 |
| URI: | http://eprints.um.edu.my/id/eprint/45675 |
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