Teoh, Teow Chong and Rizman-Idid, Mohammed and Wong, Hao Jie and Mohamad-Fauzi, Nuradilla and Abu Bakar, Nurlizah and Smykla, Jerzy and Alias, Siti Aisyah (2024) Transcriptomic Evidence of Base and Nucleotide Excision Repair Mechanisms in Response to UV-B Radiation in an Arctic Fungus Pseudogymnoascus australis Strain HNDR4. Chiang Mai Journal of Science, 51 (2). e2024029. ISSN 0125-2526, DOI https://doi.org/10.12982/CMJS.2024.029.
Full text not available from this repository.Abstract
Polar micro -fungi are known to have developed protective mechanisms and responses against the harmful effects of UV radiation. In this study, physiological, cellular, and transcriptomic responses of an Arctic fungal strain of Pseudogymnoascus australis towards UV -B radiation were examined. Fungal isolate was cultured on 0.45 mu m nylon membranes overlayed on Czapex-Dox agar (CDA) at 15 degrees C for 10 d in dark condition to reach the mid -log phase. To assess DNA damage and recovery, the isolates were exposed to UV -B radiation (UV dosage of 6.1 kj m-2 d-1) for 130 min, followed by 0, 2 and 6 hr incubation under light condition. Differential gene expression in DNA repair after UV -B treatment at these time points was analysed based on RNA-Seq data output of 40 million reads per sample from an Illumina NovaSeq 6000 system platform. The results revealed that radiation -repair (RAD) genes were upregulated and the photoreactivation (PHR1) gene was downregulated after UV -B exposure in light condition. Downregulation of oxidoreductase activity was observed from the GO enrichment analysis. The KEGG pathway enrichment analysis exhibited upregulation of base excision repair (BER) genes and nucleotide excision repair (NER) genes upon UV -B exposure. This is the first RNASeq transcriptomic study on the DNA repair mechanisms employed by a polar fungus to mitigate UV -induced DNA damage.
| Item Type: | Article |
|---|---|
| Funders: | Malaysian Ministry of Higher Education (MOHE) Higher Centre of Excellence (HiCoE) Grant (IOES-2023E); (IOES-2014G), Malaysian Ministry of Higher Education (MOHE) TRGS Grant (TR001B-2014B); (FP1213E036) |
| Uncontrolled Keywords: | base excision repair (BER); nucleotide excision repair (NER); polar micro-fungi; transcriptome; RNA-Seq |
| Subjects: | Q Science > Q Science (General) |
| Divisions: | Institute of Advanced Studies Deputy Vice Chancellor (Research & Innovation) Office > Institute of Ocean and Earth Sciences Universiti Malaya |
| Depositing User: | Ms. Juhaida Abd Rahim |
| Date Deposited: | 28 Oct 2024 01:25 |
| Last Modified: | 28 Oct 2024 01:25 |
| URI: | http://eprints.um.edu.my/id/eprint/45531 |
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