Ahmed, Khaled Abdul-Aziz and Jabbar, Ahmed A.J. and Galali, Yaseen and M. Al-Qaaneh, Ayman and Akcakavak, Gokhan and Salehen, Nur Ain and Hassan, Rawaz Rizgar and Mothana, Ramzi A. and Abdulla, Mahmood Ameen and Fantoukh, Omer I. and Hasson, Sidig and Hawwal, Mohammed F. (2024) Cumin (Cuminum cyminum L.) seeds accelerates wound healing in rats: Possible molecular mechanisms. Skin Research and Technology, 30 (5). e13727. ISSN 0909-752X, DOI https://doi.org/10.1111/srt.13727.
Full text not available from this repository.Abstract
Wound healing is a complex, intricate, and dynamic process that requires effective therapeutic management. The current study evaluates the wound healing potentials of methanolic extract of Cuminum cyminum L. seeds (CCS) in rats. Sprague Dawley (24) rats were distributed into four cages, wounds produced on the back of the neck, and received two daily topical treatments for 14 days: A, rats received normal saline; B, wounded rats treated with intrasite gel; C and D, rats received 0.2 mL of 250 and 500 mg/kg of CCS, respectively. After that, wound area and closure percentage were evaluated, and wound tissues were dissected for histopathological, immunohistochemical, and biochemical examinations. Acute toxicity trials of methanolic extract of CCS showed the absence of any physiological changes or mortality in rats. CCS application caused a significant reduction in wound size and a statistically elevated percentage of wound contraction than those of vehicle rats. CCS treatment caused significant up-regulation of collagen fiber, fibroblasts, and fewer inflammatory cells (inflammation) in granulation tissues. TGF-beta 1 (angiogenetic factor) was significantly more expressed in CCS-treated rats in comparison to normal saline-treated rats; therefore, more fibroblasts transformed into myofibroblasts (angiogenesis). CCS-treated rats showed remarkable antioxidant potentials (higher SOD and CAT enzymes) and decreased MDA (lipid peroxidation) levels in their wound tissue homogenates. Hydroxyproline amino acid (collagen) was significantly up-regulated by CCS treatment, which is commonly related to faster wound closure area. The outcomes suggest CCS as a viable new source of pharmaceuticals for wound treatment.
| Item Type: | Article |
|---|---|
| Funders: | King Saud University (RSP2023R119) |
| Uncontrolled Keywords: | antioxidants; Cuminum cyminum L.; histology; immunohistochemistry; wound |
| Subjects: | R Medicine |
| Divisions: | Faculty of Medicine > Biomedical Science Department |
| Depositing User: | Ms. Juhaida Abd Rahim |
| Date Deposited: | 30 Sep 2024 04:09 |
| Last Modified: | 30 Sep 2024 04:09 |
| URI: | http://eprints.um.edu.my/id/eprint/45253 |
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