Minaguchi, Kiyoshi and Samejima, Michinaga and Nambiar, Phrabhakaran and Kaneko, Yu and Ochiai, Eriko and Kakimoto, Yu and Osawa, Motoki (2024) Construction of 13-locus X-STR multiplex PCR system that reinforces Argus X-12 kit discrimination capacity: Application to the Malay population. Legal Medicine, 70. p. 102463. ISSN 1344-6223, DOI https://doi.org/10.1016/j.legalmed.2024.102463.
Full text not available from this repository.Abstract
Closely linked groups of markers on the X chromosome are very useful for testing complex kinship relationships involving X-STR transmission. The Argus X-12 kit, a unique commercially available kit, can obtain haplotypes of 4 linkage groups (LGs) consisting of 3 markers. Although many population data have been reported for forensic purposes, differences in discrimination ability exist between LG1 and LG2, 3, and 4 in East Asian populations, and the data of this kit would become more useful if the discrimination ability of the latter groups were increased. Therefore, for matches found using this kit for some linkage group data, then to increase the identification ability, we additionally introduced 13 X-STR loci and established a method allowing comparison using data from 25 loci. The 13X-STRs add two locus data to each of LG2, 3, and 4, and also add two closely linked group (CLG) data between LG2 and 3 and LG3 and 4 in one multiplex PCR. Assessment of this method for a Malay population for which data by Argus X-12 had already been reported showed that the frequencies of distinct haplotypes in LG2, 3, and 4 were increased by 33.0-42.6 %, and frequencies of unique haplotypes increased by 45.4-59.2 %. The respective haplotype diversity values of the additional 3-locus and 4-locus CLGs were 0.9838 and 0.9939, which helps to improve discriminatory power and to predict recombination locations on the X chromosome. Although we have been testing these loci with Japanese subjects, this system would also be useful for the Malay population.
| Item Type: | Article |
|---|---|
| Funders: | Ministry of Education, Culture, Sports, Science and Technology, Japan (MEXT) Japan Society for the Promotion of Science Grants-in-Aid for Scientific Research (KAKENHI) (JP15K11455) |
| Uncontrolled Keywords: | X -STR transmission; Argus X-12; 13-plex X -STR system; Closely linked group; X -STR haplotype data; Malay population |
| Subjects: | R Medicine |
| Divisions: | Faculty of Dentistry > Department of Oral & Maxillofacial Clinical Sciences |
| Depositing User: | Ms. Juhaida Abd Rahim |
| Date Deposited: | 17 Sep 2024 02:32 |
| Last Modified: | 17 Sep 2024 02:32 |
| URI: | http://eprints.um.edu.my/id/eprint/45097 |
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