Mararajah, Selvakumar and Giribabu, Nelli and Salleh, Naguib (2024) Chlorophytum borivilianum aqueous root extract prevents deterioration of testicular function in mice and preserves human sperm function in hydrogen peroxide (H2O2)-induced oxidative stress. Journal of Ethnopharmacology, 318 (B). ISSN 0378-8741, DOI https://doi.org/10.1016/j.jep.2023.117026.
Full text not available from this repository.Abstract
Ethnopharmacological relevance: Chlorophytum borivilianum (C. borivilianum) (CB) has traditionally been used to treat male sexual dysfunctions and has been claimed to possess aphrodisiac properties. Aim of the study: To investigate the ability of CB to ameliorate H2O2-induced oxidative stress in testes and sperm in mice and prevent H2O2-induced oxidative in human sperm. Materials and methods: Oxidative stress was induced in male mice by pre-exposure to 2% H2O2 orally for seven consecutive days, followed by 100 and 200 mg/kg b. w. administration. CB for another seven days. At the end of treatment, mice were sacrificed and testes and epididymal sperm were harvested. Serum FSH, LH and testosterone levels were measured and sperm parameters were obtained. Meanwhile, oxidative stress levels in mice testes and sperm, steroidogenesis and spermatogenesis markers in mice testes were assessed by molecular biological techniques. In another experiment, sperm from thirty-two healthy fertile men were incubated with 200 & mu;M H2O2 and CB (100 and 200 & mu;g/ml) simultaneously and were then evaluated for sperm parameter changes. Results: In mice, CB administration ameliorates persistent increases in oxidative stress and decreases in antioxidative enzyme levels in testes and sperm following H2O2 pre-exposure. Additionally, CB also helps to ameliorate deterioration in sperm parameters and testicular steroidogenesis and spermatogenesis and restores the serum FSH, LH and testosterone levels near normal in mice. In humans, CB helps to prevent deterioration in sperm parameters following H2O2 exposure. Conclusion: CB is potentially useful to preserve the male reproductive capability and subsequently male fertility in high oxidative stress conditions.
| Item Type: | Article |
|---|---|
| Funders: | UMSC Care Research Fund |
| Uncontrolled Keywords: | Chlorophytum borivilianum; Testes; Sperm |
| Subjects: | R Medicine > RG Gynecology and obstetrics |
| Divisions: | Faculty of Medicine > Physiology Department Centre for Natural Products Research and Drug Discovery (CENAR) |
| Depositing User: | Ms. Juhaida Abd Rahim |
| Date Deposited: | 05 Jul 2024 07:57 |
| Last Modified: | 05 Jul 2024 07:57 |
| URI: | http://eprints.um.edu.my/id/eprint/44324 |
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