Shaz, Norshazliza and Maran, Sathiya and Genasan, Krishnamurithy and Choudhary, Rajan and Alias, Rodianah and Swamiappan, Sasikumar and Kamarul, Tunku and Raghavendran, Hanumanth Rao Balaji (2024) Functionalization of poly (lactic-co-glycolic acid) nano-calcium sulphate and fucoidan 3D scaffold using human bone marrow mesenchymal stromal cells for bone tissue engineering application. International Journal of Biological Macromolecules, 256 (1). ISSN 0141-8130, DOI https://doi.org/10.1016/j.ijbiomac.2023.128059.
Full text not available from this repository.Abstract
This study aimed to functionalize a novel porous PLGA (Poly lactic-co-glycolic acid) composite scaffold in combination with nano-calcium sulphate (nCS) and/or fucoidan (FU) to induce osteogenic differentiation of human bone marrow stromal cells. The composite scaffolds (PLGA-nCS-FU, PLGA-nCS or PLGA-FU) were fabricated and subjected to characterization using Fourier-transform infrared spectroscopy (FTIR), X-ray powder diffraction (XRD), Scanning electron microscopy (SEM) and Energy Dispersive X-Ray (EDX). The biocompatibility and osteogenic induction potential of scaffolds on seeded human bone marrow derived mesenchymal stromal cells (hBMSCs) were studied using cell attachment and alamar blue cell viability and alkaline phosphatase (ALP), osteocalcin and osteogenic gene expression, respectively. The composition of different groups was reflected in FTIR, XRD and EDX. The SEM micrographs revealed a difference in the surface of the scaffold before and after FU addition. The confocal imaging and SEM micrographs confirmed the attachment of cells onto all three composite scaffolds. However, the AB assay indicated a significant increase (p < 0.05) in cell viability/ proliferation seeded on PLGA-nCS-FU on day 21 and 28 as compared with other combinations. A 2-fold significant increase (p < 0.05) in ALP and OC secretion of seeded hBMSCs onto PLGA-nCS-FU was observed when compared with other combinations. A significant increase in RUNX2, OPN, COL-I and ALP genes were observed in the cells seeded on PLGA-nCS-FU on day 14 and 28 as compared with day 0. In conclusion, the incorporation of both Fucoidan and Nano-calcium sulphate with PLGA showed a promising improvement in the osteogenic potential of hBMSCs. Therefore, PLGA-nCS-FU could be the ideal candidate for subsequent pre-clinical studies to develop a successful bone substitute to repair critical bone defects.
| Item Type: | Article |
|---|---|
| Funders: | UMSC CA.R.E fund [PV045-2021], Monash University Internal Grant [ECR-000002] |
| Uncontrolled Keywords: | Gellan; Chitosan; Bone; Calcium silicate; Osteoinduction; Bone tissue engineering |
| Subjects: | R Medicine > RB Pathology |
| Divisions: | Faculty of Medicine > Orthopaedic Surgery Department Faculty of Medicine > Physiology Department |
| Depositing User: | Ms. Juhaida Abd Rahim |
| Date Deposited: | 01 Jul 2024 02:58 |
| Last Modified: | 01 Jul 2024 02:58 |
| URI: | http://eprints.um.edu.my/id/eprint/44277 |
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