Ihsan, N. S. Mohd Nor and Sani, S. F. Abdul and Looi, Lai Meng and Pathmanathan, Dharini and Cheah, Phaik Leng and Chiew, S. F. and Bradley, D. A. (2024) EDXRF and the relative presence of K, Ca, Fe and as in amyloidogenic tissues. SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY, 308. ISSN 1873-3557, DOI https://doi.org/10.1016/j.saa.2023.123743.
Full text not available from this repository.Abstract
Trace and minor elements play crucial roles in a variety of biological processes, including amyloid fibrils for-mation. Mechanisms include activation or inhibition of enzymatic reactions, competition between elements and metal proteins for binding positions, also changes to the permeability of cellular membranes. These may influ-ence carcinogenic processes, with trace and minor element concentrations in normal and amyloid tissues potentially aiding in cancer diagnosis and etiology. With the analytical capability of the spectroscopic technique X-ray fluorescence (XRF), this can be used to detect and quantify the presence of elements in amyloid charac-terization, two of the trace elements known to be associated with amyloid fibrils. In present work, involving samples from a total of 22 subjects, samples of normal and amyloid-containing tissues of heart, kidney, thyroid, and other tissue organs were obtained, analyzed via energy-dispersive X-ray fluorescence (EDXRF). The elemental distribution of potassium (K), calcium (Ca), arsenic (As), and iron (Fe) was examined in both normal and amyloidogenic tissues using perpetual thin slices. In amyloidogenic tissues the levels of K, Ca, and Fe were found to be less than in corresponding normal tissues. Moreover, the presence of As was only observed in amyloidogenic samples; in a few cases in which there was an absence of As, amyloid samples were found to contain Fe. Analysis of arsenic in amyloid plaques has previously been difficult, often producing contradictory results. Using the present EDXRF facility we could distinguish between amyloidogenic and normal samples, with potential correlations in respect of the presence or concentration of specific elements.
| Item Type: | Article |
|---|---|
| Funders: | Fundamental Research Grant Scheme (FRGS) of the Ministry of Education Malaysia [FRGS/1/2020/STG05/UM/02/524] |
| Uncontrolled Keywords: | Amyloid fibrils; Human tissue; EDXRF; Arsenic; Elemental composition |
| Subjects: | R Medicine > RB Pathology |
| Divisions: | Faculty of Medicine > Pathology Department Faculty of Science > Institute of Mathematical Sciences Faculty of Science > Department of Physics |
| Depositing User: | Ms. Juhaida Abd Rahim |
| Date Deposited: | 25 Jun 2024 06:48 |
| Last Modified: | 25 Jun 2024 06:48 |
| URI: | http://eprints.um.edu.my/id/eprint/44228 |
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