Latif, Eira Nurfarisha Mohd and Noordin, Naqib Rafieqin and Shahari, Shahhaziq and Amir, Amirah and Lau, Yee-Ling and Cheong, Fei-Wen and Abdullah, Mohd Lutfi and Fong, Mun Yik (2024) Genetic polymorphism and clustering of the Plasmodium cynomolgi Duffy binding protein 1 region II of recent macaque isolates from Peninsular Malaysia. Parasitology Research, 123 (1). ISSN 0932-0113, DOI https://doi.org/10.1007/s00436-024-08125-0.
Full text not available from this repository.Abstract
Plasmodium cynomolgi is a simian malaria parasite that has been increasingly infecting humans. It is naturally present in the long-tailed and pig-tailed macaques in Southeast Asia. The P. cynomolgi Duffy binding protein 1 region II PcDBP1(II)] plays an essential role in the invasion of the parasite into host erythrocytes. This study investigated the genetic polymorphism, natural selection and haplotype clustering of PcDBP1(II) from wild macaque isolates in Peninsular Malaysia. The genomic DNA of 50 P. cynomolgi isolates was extracted from the macaque blood samples. Their PcDBP1(II) gene was amplified using a semi-nested PCR, cloned into a plasmid vector and subsequently sequenced. The polymorphism, natural selection and haplotypes of PcDBP1(II) were analysed using MEGA X and DnaSP ver.6.12.03 programmes. The analyses revealed high genetic polymorphism of PcDBP1(II) (pi= 0.026 +/- 0.004; Hd = 0.996 +/- 0.001), and it was under purifying (negative) selection. A total of 106 haplotypes of PcDBP1(II) were identified. Phylogenetic and haplotype analyses revealed two groups of PcDBP1(II). Amino acid length polymorphism was observed between the groups, which may lead to possible phenotypic difference between them.
| Item Type: | Article |
|---|---|
| Funders: | Ministry of Education, Malaysia [W-00256-15-19] |
| Uncontrolled Keywords: | Plasmodium cynomolgi; Duffy binding protein 1; Genetic polymorphism; Haplotypes; Natural selection; Peninsular Malaysia |
| Subjects: | Q Science > QR Microbiology R Medicine > R Medicine (General) |
| Divisions: | Faculty of Medicine > Parasitology Deparment |
| Depositing User: | Ms. Juhaida Abd Rahim |
| Date Deposited: | 20 Jun 2024 01:52 |
| Last Modified: | 20 Jun 2024 01:52 |
| URI: | http://eprints.um.edu.my/id/eprint/44198 |
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