Ahmed, Khaled Abdul-Aziz and Jabbar, Ahmed A. J. and Abdulla, Mahmood Ameen and Alamri, Zaenah Zuhair and Salehen, Nur Ain and Ibrahim, Ibrahim Abdel Aziz and Almaimani, Ghassan and Bamagous, Ghazi A. and Almaimani, Riyad A. and Almasmoum, Hussain A. and Ghaith, Mazen M. and Farrash, Wesam F. (2024) Mangiferin (mango) attenuates AOM-induced colorectal cancer in rat's colon by augmentation of apoptotic proteins and antioxidant mechanisms. Scientific Reports, 14 (1). ISSN 2045-2322, DOI https://doi.org/10.1038/s41598-023-50947-y.
Full text not available from this repository.Abstract
Mangiferin (MF) is a natural C-glucosylxantone compound that has many substantial curative potentials against numerous illnesses including cancers. The present study's goal is to appraise the chemo preventive possessions of MF on azoxymethane (AOM)-mediated colonic aberrant crypt foci (ACF) in rats. Rats clustered into 5 groups, negative control (A), inoculated subcutaneously with normal saline twice and nourished on 0.5% CMC; groups B-E injected twice with 15 mg/kg azoxymethane followed by ingestion of 0.5% CMC (B, cancer control); intraperitoneal inoculation of 35 mg/kg 5-fluorouracil (C, reference rats) or nourished on 30 mg/kg (D) and 60 mg/kg (E) of MF. Results of gross morphology of colorectal specimens showed significantly lower total colonic ACF incidence in MF-treated rats than that of cancer controls. The colon tissue examination of cancer control rats showed increased ACF availability with bizarrely elongated nuclei, stratified cells, and higher depletion of the submucosal glands compared to MF-treated rats. Mangiferin treatment caused increased regulation of pro-apoptotic (increased Bax) proteins and reduced the beta-catenin) proteins expression. Moreover, rats fed on MF had significantly higher glutathione peroxidase (GPx), superoxide dismutase (SOD), catalase (CAT), and lower malondialdehyde (MDA) concentrations in their colonic tissue homogenates. Mangiferin supplementation significantly down-shifted pro-inflammatory cytokines (transforming growth factor-alpha and interleukine-6) and up-shifted anti-inflammatory cytokines (interleukine-10) based on serum analysis. The chemo-protective mechanistic of MF against AOM-induced ACF, shown by lower ACF values and colon tissue penetration, could be correlated with its positive modulation of apoptotic cascade, antioxidant enzymes, and inflammatory cytokines originating from AOM oxidative stress insults.
| Item Type: | Article |
|---|---|
| Funders: | UNSPECIFIED |
| Uncontrolled Keywords: | INFLAMMATION; PATHWAYS; DISEASE |
| Subjects: | Q Science > QR Microbiology R Medicine > R Medicine (General) |
| Divisions: | Faculty of Medicine > Biomedical Science Department |
| Depositing User: | Ms. Juhaida Abd Rahim |
| Date Deposited: | 14 Jun 2024 06:01 |
| Last Modified: | 14 Jun 2024 06:01 |
| URI: | http://eprints.um.edu.my/id/eprint/44173 |
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