Safari, Sedigheh and Eidi, Akram and Mehrabani, Mehrnaz and Fatemi, Mohammad Javad and Sharifi, Ali Mohammad (2022) Conditioned medium of adipose-derived mesenchymal stem cells as a promising candidate to protect high glucose-induced injury in cultured C28I2 chondrocytes. Advanced Pharmaceutical Bulletin, 12 (3). 632 – 640. ISSN 2228-5881, DOI https://doi.org/10.34172/apb.2022.044.
Full text not available from this repository.Abstract
Purpose: The aim of this study was to evaluate the protective effect of conditioned medium derived from human adipose mesenchymal stem cells (CM-hADSCs) on C28I2 chondrocytes against oxidative stress and mitochondrial apoptosis induced by high glucose (HG). Methods: C28I2 cells were pre-treated with CM-hADSCs for 24 hours followed by HG exposure (75 mM) for 48 hours. MTT assay was used to assess the cell viability. Reactive oxygen species (ROS) and lipid peroxidation were determined by 2,7-dichlorofluorescein diacetate (DCFH-DA) and thiobarbituric acid reactive substances (TBARS) assays, respectively. Expressions of glutathione peroxidase 3 (GPX 3), heme oxygenase-1 (HO-1), and NAD(P)H quinone dehydrogenase 1 (NQO1) were analyzed by RT-PCR. Finally, western blot analysis was used to measure Bax, Bcl-2, cleaved caspase-3, and Nrf-2 expression at protein levels. Results: CM-hADSCs pretreatment mitigated the cytotoxic effect of HG on C28I2 viability. Treatment also markedly reduced the levels of ROS, lipid peroxidation, and augmented the expression of HO-1, NQO1, and GPx3 genes in HG-exposed group. CM-ADSCs enhanced Nrf-2 protein expression and reduced mitochondrial apoptosis through reducing Bax/Bcl-2 ratio and Caspase-3 activation. Conclusion: MSCs, probably through its paracrine effects, declined the deleterious effect of HG on chondrocytes. Hence, therapies based on MSCs secretomes appear to be a promising therapeutic approaches to prevent joint complications in diabetic patients. © 2022 The Author (s).
| Item Type: | Article |
|---|---|
| Funders: | National Institute for Medical Research Development |
| Uncontrolled Keywords: | Adipose derived mesenchymal stem cells; Conditioned medium; High glucose; Oxidative stress |
| Subjects: | R Medicine R Medicine > RS Pharmacy and materia medica |
| Divisions: | Faculty of Medicine > Orthopaedic Surgery Department |
| Depositing User: | Ms. Juhaida Abd Rahim |
| Date Deposited: | 31 Jan 2024 03:32 |
| Last Modified: | 26 Jun 2024 01:37 |
| URI: | http://eprints.um.edu.my/id/eprint/43903 |
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