Kamaruzzaman, Alya Nur Athirah and Mulok, Tengku Elida Tengku Zainal and Nor, Nurul Hidayah Mohamad and Yahya, Mohd Fakharul Zaman Raja (2022) Ftir spectral changes in candida albicans biofilm following exposure to antifungals. Malaysian Applied Biology, 51 (4). 57 – 66. ISSN 0126-8643, DOI https://doi.org/10.55230/mabjournal.v51i4.11.
Full text not available from this repository.Abstract
Candida albicans is a microbial fungus that exists as a commensal member of the human microbiome and an opportunistic pathogen. Biofilm formation by this fungal pathogen occurs mostly in the mucosa or endothelium associated with candidiasis and colonizes medical devices. The present work was performed to determine the efficacy of the antifungal creams on the viability and biochemical composition of C. albicans biofilm. Four commercial antifungal creams were used herein namely econazole nitrate, miconazole nitrate, ketoconazole and tolnaftate. Resazurin assay and Fourier transform infrared (FTIR) spectroscopy were performed to determine the viability and biochemical composition of C. albicans biofilm, respectively. Results demonstrated that the antifungal creams inhibited C. albicans biofilm. The highest percent inhibition shown by econazole nitrate, miconazole nitrate, ketoconazole, and tolnaftate were 16.5, 17.1, 15.8, and 6.9, respectively. Econazole nitrate with the lowest IC50 value of 43.42 µg/mL caused changes in the FTIR spectral peak shape at 1377 cm-1 and 1736 cm-1. On the other hand, miconazole nitrate with the second lowest IC50 value of 118.26 µg/mL caused spectral peak shifting from 1237 cm-1 to 1228 cm-1. In conclusion, the inhibition of C. albicans biofilm may be mediated by the changes in protein, lipid, and nucleic acid compositions. © 2022, Malaysian Society of Applied Biology. All rights reserved.
| Item Type: | Article |
|---|---|
| Funders: | UNSPECIFIED |
| Additional Information: | Cited by: 2; All Open Access, Bronze Open Access, Green Open Access |
| Uncontrolled Keywords: | Antibiofilm; Biofilm formation; Candida; FTIR spectrosco; Fungal pathogen |
| Subjects: | Q Science > Q Science (General) Q Science > QC Physics |
| Divisions: | Faculty of Science Faculty of Science > Department of Physics |
| Depositing User: | Ms. Juhaida Abd Rahim |
| Date Deposited: | 18 Nov 2024 04:49 |
| Last Modified: | 18 Nov 2024 04:49 |
| URI: | http://eprints.um.edu.my/id/eprint/43850 |
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