Kwa, Yee Chu and Anasamy, Theebaa and Foo, Yiing Yee and Leo, Bey Fen and Chung, Ivy and Kiew, Lik Voon and Chung, Lip Yong (2022) Preparation and characterization of stattic-loaded albumin nanoparticles for antimetastatic cancer treatment. Drug Delivery Letters, 12 (1). 74 – 83. ISSN 22103031, DOI https://doi.org/10.2174/2210303112666220330115110.
Full text not available from this repository.Abstract
Background: Stattic offers a unique inhibitory effect on the STAT3 signaling pathway, a crucial mechanism in the progression of metastatic cancer. However, the development of Stattic has been impeded by its hydrophobicity and lack of specificity. To overcome these limitations, encapsulation of Stattic with polymeric micelles was previously attempted, which led to a significant increase in the potency of Stattic on breast cancer cell lines. The presence of albumin was believed to contribute to such enhancement, as the protein corona layer formation helps retain the micellar structure before eventual uptake by the cells. Moreover, a previous study had reported the unique affinity of Stattic towards albumin molecule. Objective: This study aimed to explore the integration of Stattic in albumin-based nanoparticles and to assess the in vitro effects. Methods: Albumin/Stattic nanoparticles were prepared by crosslinking with glutaraldehyde. Results: The yielded nanoparticles were 150.0 ± 6.6 nm in size, with ~53 entrapment efficiency. he cumulative release of Stattic in a tumoric acidic environment (pH 5.3; 59) was 2.6-fold more than neutral environment (pH 7.4; 23). In blood plasma, 7 cumulative release was observed. The mathematical modeling of the release kinetics revealed that the albumin/Stattic nanoparticles in phosphate buffer saline and plasma followed Korsmeyer-Peppas and Higuchi models, respectively. Among the two cell lines tested, metastatic MDA-MB-231 cells were more sensitive to entrapment of Stattic with albumin nanoparticles, as the IC50 value decreased by 2.5-fold compared to free Stattic. Conclusion: This study reports the formation of low immunogenic and cost-efficient albumin nanoparticles to improve the delivery of Stattic. © 2022 Bentham Science Publishers.
| Item Type: | Article |
|---|---|
| Funders: | UNSPECIFIED |
| Additional Information: | Cited by: 0 |
| Uncontrolled Keywords: | Albumin; Benzothiophene; Bovine serum albumin; Glutaraldehyde; Nanocarrier; Nanoparticle; Stattic; Stattic loaded albumin nanoparticle; unclassified drug; Article; breast cancer; cancer therapy; chemical parameters; controlled study; cross linking; cytotoxicity; cytotoxicity assay; drug analysis; drug delivery system; drug release; drug synthesis; electrophoretic mobility; entrapment efficiency; high performance liquid chromatography; higuchi model; hixson crowell model; human; human cell; IC50; kinetics; korsmeyer peppas model; mathematical model; MCF-7 cell line; MDA-MB-231 cell line; metastasis; metastasis inhibition; model; photon correlation spectroscopy; transmission electron microscopy; ultraviolet visible spectroscopy; zeta potential |
| Subjects: | R Medicine R Medicine > RM Therapeutics. Pharmacology R Medicine > RS Pharmacy and materia medica |
| Divisions: | Faculty of Medicine Faculty of Medicine > Medicine Department |
| Depositing User: | Ms. Juhaida Abd Rahim |
| Date Deposited: | 14 Nov 2024 04:38 |
| Last Modified: | 14 Nov 2024 04:38 |
| URI: | http://eprints.um.edu.my/id/eprint/43597 |
Actions (login required)
 |
View Item |
