Wahyuningsih, Sri Puji Astuti and Dewi, Firli Rahmah Primula and Hsan, Amy Saik Yi and Lee, Looi Mee and Lim, Vuanghao and Aun, Lionel In Lian and Ling, Tau Chuan and Marviella, Sephia Tiara (2022) The regulation of Hypoxia Inducible Factor (HIF)1α expression by quercetin: An in silico study. Acta Informatica Medica, 30 (2). 96 – 99. ISSN 0353-8109, DOI https://doi.org/10.5455/aim.2022.30.96-99.
Full text not available from this repository.Abstract
Background: Cancer disease is a growing health problem in developing and developed countries. Hypoxia-inducible factor-1a (HIF1α) is a transcription factor responsible for expressing several proteins involved in angiogenesis. Quercetin can suppress HIF1α expression due to the inhibition of protein synthesis. However, to date, the study exploring the potential of quercetin in repressing HIF1α through its degradation mechanism has never been done. An in silico study is needed as a preliminary study to understand the mechanism underlining this possibility. Objective: This study aimed to investigate the potential of quercetin in regulating HIF1α expression through the ubiquitin degradation pathway by in silico study. Methods: This study was performed by in silico analysis, including biological activity prediction, 3D protein structure collection, protein-ligand and protein-protein docking, and the visualization of the docking results. Results: The probability activity (Pa) score of quercetin as an HIF1α expression inhibitor was 0.969. In the absence of quercetin, the center-weighted score of HIF1α - pVHL, HIF1α - FIH, and HIF1α - PHD2 was -699.4 kJ/mol, -846.0 kJ/mol, and -650.5 kJ/mol, respectively. In the presence of quercetin, the weighted score of HIF1α - pVHL, HIF1α - FIH, and HIF1α - PHD2 was reduced to -728.1 kJ/mol, -854.2 kJ/mol, and -650.5 kJ/mol, respectively. Conclusion: Quercetin could directly promote HIF1α and pVHL interaction, thus increasing the degradation of HIF1α by ubiquitin-dependent pathway. © 2022 Sri Puji Astuti Wahyuningsih, Amy Saik Yi Hsan, Looi Mee Lee, Vuanghao Lim, Lionel In Lian Aun, Sephia Tiara Marviella, Firli Rahmah Primula Dewi.
| Item Type: | Article |
|---|---|
| Funders: | Universitas Airlangga [Grant no. 1301/UN3.15/PT/2021] |
| Uncontrolled Keywords: | Docking protein; Hypoxia inducible factor 1alpha; Quercetin; ubiquitin; Biological activity; Computer model; Human; Protein expression; Protein structure |
| Subjects: | Q Science > QH Natural history > QH301 Biology R Medicine |
| Divisions: | Faculty of Medicine > Biomedical Science Department Faculty of Science > Institute of Biological Sciences |
| Depositing User: | Ms. Juhaida Abd Rahim |
| Date Deposited: | 04 Nov 2025 01:03 |
| Last Modified: | 04 Nov 2025 01:03 |
| URI: | http://eprints.um.edu.my/id/eprint/43591 |
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