An in vitro study to evaluate the genotoxicity of valueadded hydroxyapatite as a bone replacement material

Hassan, A. and Swaminathan, D. (2011) An in vitro study to evaluate the genotoxicity of valueadded hydroxyapatite as a bone replacement material. Sains Malaysiana, 40 (2). pp. 163-171. ISSN 0126-6039

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Abstract

Hydroxyapatite (HA) used for bone replacement is one of the most active areas of ceramic biomaterials research currently. It has been used clinically for the last 20 years due to its excellent biocompatibility, osseoconduction and osseointegration. Many modifications have been done to develop a stronger, tougher and biocompatible ceramic biomaterial because pure HA is brittle. Researchers in Universiti Sains Malaysia had developed this value added HA that is stronger and less brittle compared to pure HA. The objective of this in vitro study was to evaluate the genotoxic characteristic of the value added HA based material by using Bacterial Reverse Mutation Assay (Ames test). The Bacterial Reverse Mutation Assay of HA was performed on Salmonella typhimurium strains TA98, TA100, TA1535, TA1537 and Escherichia coli strain WP2 uvrA using the preincubation method in the presence and absence of an exogenous metabolic activation system. All the bacterial tester strains treated with and without S9 Mix showed no increase of revertant colonies with increase in concentration of test substance for both the dose finding test and the main test. The number of revertant colonies was less than twice that of the solvent control for all the jive bacterial strains and this was reproducible for both the dose finding test and the main test. The numbers of revertant colonies in the negative and positive controls were within the background data of our laboratory. In conclusion the results of the tests showed that the value added HA was considered to have no reverse mutagenic potential under the present test conditions.

Item Type: Article
Additional Information: ISI Document Delivery No.: 727SJ Times Cited: 0 Cited Reference Count: 16 Cited References: ANDERSON JC, 1990, MAT SCI, P317 BAJPAI PK, 1976, CLIN RES, V24, pA524 Chauvel-Lebret DJ, 2001, BIOMATERIALS, V22, P1795, DOI 10.1016/S0142-9612(00)00351-3 COSTANTINO PD, 1991, ARCH OTOLARYNGOL, V117, P379 FERRARO JW, 1979, PLAST RECONSTR SURG, V63, P634 JARCHO M, 1981, CLIN ORTHOP RELAT R, P259 JOHNSON GJ, 1998, PREVALENCE INCIDENCE, P1 Katzer A, 2002, BIOMATERIALS, V23, P1749, DOI 10.1016/S0142-9612(01)00300-3 Li J., 1993, Clinical Materials, V12, P197, DOI 10.1016/0267-6605(93)90073-G MEFFERT RM, 1985, J PERIODONTOL, V56, P63 Mortelmans K, 2000, MUTAT RES-FUND MOL M, V455, P29, DOI 10.1016/S0027-5107(00)00064-6 Piconi C, 1999, BIOMATERIALS, V20, P1, DOI 10.1016/S0142-9612(98)00010-6 SMITH WF, 1996, PRINCIPLES MAT SCI E, P633 WILLIAMS DF, 1987, PROGR BIOMEDICAL ENG, V4, P54 *ISO, 1992, BIOL EV MED DEV TEST, P1 *OECD, 1997, TOW SUST FISH, P1 Hassan, Akram Swaminathan, Dasan Univ kebangsaan malaysia Kuala lumpur
Uncontrolled Keywords: Ames test genotoxicity hydroxyapatite calcium-phosphate ceramics assay
Subjects: R Medicine > RK Dentistry
Divisions: Faculty of Dentistry > Dept of Oral Pathology & Oral Medicine & Periodontology
Depositing User: Ms Nursyafiqah Abd Malek
Date Deposited: 10 Jan 2013 00:21
Last Modified: 19 Mar 2019 09:00
URI: http://eprints.um.edu.my/id/eprint/4351

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