Wan, Steffi Julan and Sulaiman, Nurshahira and Zulkifli, Nurul Farhana and Neela, Vasantha Kumari and Chee, Hui Yee and Masri, Siti Norbaya and Ikbal, Nurul Huda Mohamad and Bhassu, Subha and Omar, Hasmahzaiti and Al-Obaidi, Mazen M. Jamil and Desa, Mohd Nasir Mohd (2022) Detection of pathogenic leptospira in rats and phylogenetic analysis using outer membrane lipoprotein Lipl32 gene at two major public markets. Malaysian Journal of Medicine and Health Sciences, 18 (2). 1 – 5. ISSN 16758544,
Full text not available from this repository.Abstract
Introduction: Limited studies have been documented on the presence of pathogenic Leptospira in public markets serving the community in sub-districts of Selangor. The aim of this study was to detect the presence of pathogenic Leptospira in rats using a gene encoding an outer membrane lipoprotein LipL32. Methods: Polymerase chain reaction (PCR) was performed using LipL32 primers on sixty kidney samples of rats trapped at two locations of study; Pasar Borong Selangor in Seri Kembangan and Pasar Basah Bandar Baru Bangi in Bangi. Results: Out of 60 samples analysed, 36.7 were positive for the presence of LipL32. All positive samples highly matched (>94) nucleotide sequence for LipL32 of pathogenic Leptospira and related to the pathogens through phylogenetic analysis. Conclusion: The detection of LipL32 indicates the potential presence of pathogenic Leptospira species at public markets. Although only 60 rats were successfully trapped, the rats are mobile and might further transmit the pathogenic organisms to other areas. © 2022 UPM Press. All rights reserved.
| Item Type: | Article |
|---|---|
| Funders: | Ministry of Higher Learning, Malaysia [Grant No; 5526406] |
| Subjects: | Q Science > QH Natural history > QH301 Biology |
| Divisions: | Faculty of Science > Institute of Biological Sciences |
| Depositing User: | Ms. Juhaida Abd Rahim |
| Date Deposited: | 07 Nov 2023 02:45 |
| Last Modified: | 07 Nov 2023 02:45 |
| URI: | http://eprints.um.edu.my/id/eprint/43478 |
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