Differential expression of basement membrane collagen-IV alpha l to alpha 6 chains during oral carcinogenes

Tamamura, R.; Nagatsuka, H.; Siar, C.H.; Katase, N.; Naito, I.; Sado, Y.; Nagai, N. (2006) Differential expression of basement membrane collagen-IV alpha l to alpha 6 chains during oral carcinogenes. Virchows Archiv, 449 (3). pp. 358-366. ISSN 0945-6317

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    Abstract

    This study aimed to resolve if basement membrane (BM) collagen alpha chains undergo remodeling during oral. carcinogenesis. Using immunohistochemistry and transmission electron microscopy, we found that BMs in oral epithelial dysplasias (OED: mild, n=10; moderate, n=10; severe, n=10) and carcinoma in situ (CIS) (n=10) differed from normal mucosa (n=6) and oral epithelial hyperplasia (n=5) in showing: (1) excessive lamina densa-like material ultrastructurally, and (2) stronger immunoexpression for alpha 5(IV) than for alpha 1(IV), alpha 2(IV), and alpha 6(IV) chains-findings that implicate these molecules' role as an adhesive template for the attachment and persistence of basal dysplastic cells. Incipient loss of BM integrity in CIS, where alpha 5(IV)/alpha 6(IV) chains were more frequently absent than alpha 1(IV)/alpha 2(IV) chains, suggests that alpha(IV) network disruption is crucial for progression of dysplastic cells into the extracellular compartment, marking transition into the invasive phase. In carcinomatous BM, the disappearance of alpha(IV) chains was more severe in poorly differentiated oral squamous cell carcinoma (OSCC) (n=10) than in well-differentiated OSCC (n=10). In all samples examined, alpha 3(IV) and alpha 4(IV) chains were absent. These findings taken together suggest that BM collagen-IV alpha chains undergo remodeling where selective increase and loss of these molecules are probably early and late events, respectively, during progression of oral dysplasia to cancer.

    Item Type: Article
    Creators:
    1. Tamamura, R.
    2. Nagatsuka, H.
    3. Siar, C.H.
    4. Katase, N.
    5. Naito, I.
    6. Sado, Y.
    7. Nagai, N.
    Journal or Publication Title: Virchows Archiv
    Additional Information: ISI Document Delivery No.: 096NV Times Cited: 2 Cited Reference Count: 32 Cited References: Andriani F, 2004, INT J CANCER, V108, P348, DOI 10.1002/ijc.11525 Barnes LEJ, 2005, WHO CLASSIFICATION T Borza DB, 2001, J BIOL CHEM, V276, P28532, DOI 10.1074/jbc.M103690200 Boutaud A, 2000, J BIOL CHEM, V275, P30716, DOI 10.1074/jbc.M004569200 Dehan P, 1997, AM J PATHOL, V151, P1097 Erickson AC, 2000, J HISTOCHEM CYTOCHEM, V48, P1291 Firth NA, 1996, AUST DENT J, V41, P83 Fleischmajer R, 1997, J INVEST DERMATOL, V109, P527, DOI 10.1111/1523-1747.ep12336696 Floquet N, 2004, J BIOL CHEM, V279, P2091, DOI 10.1074/jbc.M307736200 Garzino-Demo P, 1998, ORAL ONCOL, V34, P204, DOI 10.1016/S1368-8375(97)00059-6 GIANNELLI G, 2001, J BIOL REGUL HOMEOST, V4, P375 Hiki Y, 2002, PATHOL INT, V52, P224, DOI 10.1046/j.1440-1827.2002.01341.x Kadono G, 2004, PANCREAS, V29, P61, DOI 10.1097/00006676-200407000-00057 Kagawa M, 1997, NEPHROL DIAL TRANSPL, V12, P1238, DOI 10.1093/ndt/12.6.1238 KANNAN S, 1994, HISTOPATHOLOGY, V24, P531 Lohi J, 1997, INT J CANCER, V72, P43, DOI 10.1002/(SICI)1097-0215(19970703)72:1<43::AID-IJC6>3.0.CO;2-4 Misumi S, 2004, VIRCHOWS ARCH, V445, P54, DOI 10.1007/s00428-004-1015-3 Nagai N, 2002, J ORAL PATHOL MED, V31, P481, DOI 10.1034/j.1600-0714.2002.00010.x Nagatsuka H, 2002, VIRCHOWS ARCH, V441, P392, DOI 10.1007/s00428-002-0658-1 Nagatsuka H, 2005, VIRCHOWS ARCH, V447, P710, DOI 10.1007/s00428-005-0016-1 Nakano K, 2001, J PATHOL, V194, P420, DOI 10.1002/path.928 Nakano K, 2002, J ORAL PATHOL MED, V31, P494, DOI 10.1034/j.1600-0714.2002.00162.x Nakano S, 1999, LAB INVEST, V79, P281 NINOMIYA Y, 1995, J CELL BIOL, V130, P1219, DOI 10.1083/jcb.130.5.1219 Ortega N, 2002, J CELL SCI, V115, P4201, DOI 10.1242/jcs.00106 Reibel J, 2003, CRIT REV ORAL BIOL M, V14, P47 SADO Y, 1995, HISTOCHEM CELL BIOL, V104, P267, DOI 10.1007/BF01464322 Seki T, 1998, HISTOCHEM CELL BIOL, V110, P359, DOI 10.1007/s004180050296 Tanaka K, 1997, HISTOCHEM J, V29, P563, DOI 10.1023/A:1026428010104 Tosios KI, 1998, HISTOPATHOLOGY, V33, P261, DOI 10.1046/j.1365-2559.1998.00452.x Wilson DF, 1999, AUST DENT J, V44, P93, DOI 10.1111/j.1834-7819.1999.tb00207.x YURCHENCO PD, 1994, CURR OPIN CELL BIOL, V6, P674, DOI 10.1016/0955-0674(94)90093-0 Tamamura, Ryo Nagatsuka, Hitoshi Siar, Chong Huat Katase, Naoki Naito, Ichiro Sado, Yoshikazu Nagai, Noriyuki Springer New york
    Uncontrolled Keywords: Collagen-IV chains epithelial dysplasia carcinoma in situ squamous cell carcinoma oral carcinogenesis defined monoclonal-antibodies cell carcinomas basal lamina tumors immunofluorescence localization progression prognosis proteins melanoma
    Subjects: R Medicine > RK Dentistry
    Divisions: Faculty of Dentistry > Dept of Oral Pathology & Oral Medicine & Periodontology
    Depositing User: Ms Nursyafiqah Abd Malek
    Date Deposited: 10 Jan 2013 08:17
    Last Modified: 10 Jan 2013 08:17
    URI: http://eprints.um.edu.my/id/eprint/4344

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