Tan, Kim-Kee and Abubakar, S. (2022) Differential heterologous neutralisation profile against strains within DENV-3 genotype II. Epidemiology and Infection, 150. ISSN 09502688, DOI https://doi.org/10.1017/S0950268821002648.
Full text not available from this repository.Abstract
The dengue virus type 3 (DENV-3) homotypic outbreak cycles reported in Klang Valley, Malaysia in 1992-1995 and 2002 demonstrated different epidemic magnitude and duration. These outbreak cycles were caused by two closely related strains of viruses within the DENV-3 genotype II (DENV-3/II). The role of viral genotypic diversity and factors that could have influenced this phenomenon were investigated. The serum neutralisation sensitivity of DEN3/II strains responsible for the DENV-3 outbreak cycles in 1992-1995 and 2002 were examined. Representative virus isolates from the respective outbreaks were subjected to virus neutralisation assay using identified sera of patients with homotypic (DENV-3) or heterotypic dengue infections (DENV-1 and DENV-2). Results from the study suggested that isolates representing DENV-3/II group E (DENV-3/II-E) from the 1992-1995 outbreak and DENV-3/II group F (DENV-3/II-F) from the 2002 outbreak were neutralised at similar capacity (intergenotypic differences <2-fold) by sera of patients infected with DENV-3, DENV-1 and DENV-2/Asian genotypes. Sera of the DENV-2/Cosmopolitan infection efficiently neutralised DENV-3/II-F (FRNT50 = 508.0) at a similar neutralisation capacity against its own homotypic serotype, DENV-2 (FRNT50 = 452.5), but not against DENV-3/II-E (FRNT50 = 100.8). The different neutralisation sensitivities of DENV-3/II strains towards the cross-reacting DENV-2 heterotypic immunity could play a role in shaping the DENV-3 recurring outbreaks pattern in Malaysia. Two genetic variations, E-132 (H/Y) and E-479 (A/V) were identified on the envelope protein of DENV-3/II-E and DENV-3/II-F, respectively. The E-132 variation was predicted to affect the protein stability. A more extensive study, however, on the implication of the naturally occurring genetic variations within closely related DENV genotypes on the neutralisation profile and protective immunity would be needed for a better understanding of the DENV spread pattern in a hyperendemic setting. Copyright © The Author(s), 2021. Published by Cambridge University Press
| Item Type: | Article |
|---|---|
| Funders: | Ministry of Higher Education, Malaysia under the Higher Institution Centre of Excellence (HICoE) program [Grant No; MO002-2019], Fundamental Research Grant Scheme [Grant No; FRGS-MRSA/1/2018/SKK08/UM/01/1 (MO012-2017)], Universiti Malaya [Grant No; RU005-2020], University of Malaya (RU SATU Joint Research grant scheme) [Grant No; ST015-2018] |
| Uncontrolled Keywords: | Antibodies, Neutralizing; Antibodies, viral; Dengue; Dengue virus; Disease outbreaks; Gene products, env; Genotype; Humans; Malaysia; Virus envelope protein; Neutralizing antibody; Virus antibody; Virus envelope protein; Article; Communicable disease; Dengue virus 1; Dengue virus 2; Dengue virus 3; Genetic variation; Genotype; Immunity; Nonhuman; Protein stability; Serotype; Virus isolation; Virus neutralization; Virus strain; Blood; Dengue; Dengue virus; Epidemic; Genetics; Human; Immunology; Malaysia; Virology |
| Subjects: | Q Science > QR Microbiology > QR355 Virology R Medicine > R Medicine (General) |
| Divisions: | Deputy Vice Chancellor (Research & Innovation) Office > Tropical Infectious Diseases Research and Education Centre |
| Depositing User: | Ms. Juhaida Abd Rahim |
| Date Deposited: | 09 Nov 2023 03:32 |
| Last Modified: | 09 Nov 2023 03:32 |
| URI: | http://eprints.um.edu.my/id/eprint/43390 |
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