Subcutaneous reactions and degradation characteristics of collagenous and noncollagenous membranes in a macaque model

Siar, C.H.; Toh, C.G.; Romanos, G.; Ng, K.H. (2011) Subcutaneous reactions and degradation characteristics of collagenous and noncollagenous membranes in a macaque model. Clinical Oral Implants Research, 22 (1). pp. 113-120. ISSN 0905-7161

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    Abstract

    Background Collagenous and noncollagenous membranes have been investigated in many animal systems but their effects in the macaque model are unknown. Objective To determine subcutaneous cellular reactions and degradation characteristics following implantation of collagenous and noncollagenous membranes in a macaque model. Methods Six adult male Macaca fascicularis, aged above 7 years, were used. Six commercially available collagenous (Bio-Gide (R) BG, Tissue Fleece (R) TFL TissueFoil E forte (R) TFO, Lycoll (R) LC, Surgicoll (R) SG and Tutodent (R) TU) and two noncollagenous (Tabotamp (R) TA and Gelita-Tampon (R) GT) membranes (size 2 x 2 cm each) were implanted in unconnected subcutaneous pouches in the monkey's back and wounds were allowed to heal by primary intention. The total sample size for each membrane was six. Two monkeys were sacrificed for each experimental period of 4, 14 and 28 days. Explanted specimens were prepared for histologic and histomorphometric analysis. Digitized images of implant sites were systematically sampled using an Image Analyzer with a grid containing 35 intersection points. Four parameters were quantified: membrane degradation, foreign body reaction, tissue organization and vascularization. Results Biodegradation rate and vascularization scored higher in collagenous than in noncollagenous membranes. Except for TFL and TU, the remaining six membranes showed a moderately intense foreign body reaction at week 2. Tissue organization was initiated early in four out of six collagenous (TFL > LC > SG > TFO > BG > TU) compared with one of two noncollagenous (TA > GT) membranes. Conclusions The results suggest that differences in membrane structure and composition underlie their different cellular reactions and degradation characteristics. To cite this article:Siar CH, Toh CG, Romanos G, Ng KH. Subcutaneous reactions and degradation characteristics of collagenous and noncollagenous membranes in a macaque model. Clin. Oral Impl. Res. 22, 2011; 113-120.doi: 10.1111/j.1600-0501.2010.01970.x.

    Item Type: Article
    Creators:
    1. Siar, C.H.
    2. Toh, C.G.
    3. Romanos, G.
    4. Ng, K.H.
    Journal or Publication Title: Clinical Oral Implants Research
    Additional Information: ISI Document Delivery No.: 695RQ Times Cited: 1 Cited Reference Count: 36 Cited References: ALPAR B, 2000, SEMIN IMMUNOL, V4, P219 Anderson JM, 2008, SEMIN IMMUNOL, V20, P86, DOI 10.1016/j.smim.2007.11.004 Bashutski JD, 2009, J ENDODONT, V35, P321, DOI 10.1016/j.joen.2008.11.023 Behring J, 2008, ODONTOLOGY, V96, P1, DOI 10.1007/s10266-008-0087-y BERGSMA JE, 1995, BIOMATERIALS, V16, P25, DOI 10.1016/0142-9612(95)91092-D Bunyaratavej P, 2001, J PERIODONTOL, V72, P215, DOI 10.1902/jop.2001.72.2.215 Gielkens PFM, 2007, INT J ORAL MAX IMPL, V22, P390 Gogolewski S, 2000, INJURY, V31, pS28 Herten M, 2009, CLIN ORAL IMPLAN RES, V20, P116, DOI 10.1111/j.1600-0501.2008.01622.x Jung RE, 2006, CLIN ORAL IMPLAN RES, V17, P426, DOI 10.1111/j.1600-0501.2005.01228.x Kasaj Adrian, 2008, Head Face Med, V4, P22, DOI 10.1186/1746-160X-4-22 Kim SH, 2009, CLIN ORAL IMPLAN RES, V20, P1124, DOI 10.1111/j.1600-0501.2009.01744.x Kofidis T, 2003, TISSUE ENG, V9, P517, DOI 10.1089/107632703322066697 Kohal RJ, 1998, J PERIODONTOL, V69, P927 Luttikhuizen DT, 2006, BIOMATERIALS, V27, P5763, DOI 10.1016/j.biomaterials.2006.07.004 Luttikhuizen DT, 2007, J BIOMED MATER RES A, V83A, P879, DOI 10.1002/jbm.a.31420 Luttikhuizen DT, 2007, J TISSUE ENG REGEN M, V1, P398, DOI 10.1002/term.50 Malicev E, 2007, BIOTECHNOL BIOENG, V96, P364, DOI 10.1002/bit.21038 Mano JF, 2007, J R SOC INTERFACE, V4, P999, DOI 10.1098/rsif.2007.0220 Mattson JS, 1999, J PERIODONTOL, V70, P510, DOI 10.1902/jop.1999.70.5.510 McAllister BS, 2007, J PERIODONTOL, V78, P377, DOI 10.1902/jop.2007.060048 MINABE M, 1989, J PERIODONTOL, V60, P35 Moses O, 2009, CLIN ORAL IMPLAN RES, V20, P189, DOI 10.1111/j.1600-0501.2008.01607.x Owens K W, 2001, Implant Dent, V10, P49 POSTLETHWAITE AE, 1978, P NATL ACAD SCI USA, V75, P871, DOI 10.1073/pnas.75.2.871 ROMANOS GE, 1998, BIOL MATRICES TISSUE, P13 ROMANOS GE, 1995, J BIOMED MATER RES, V29, P1121, DOI 10.1002/jbm.820290912 Rothamel D, 2005, CLIN ORAL IMPLAN RES, V16, P369, DOI 10.1111/j.1600-0501.2005.01108.x Rothamel D, 2004, CLIN ORAL IMPLAN RES, V15, P443, DOI 10.1111/j.1600-0501.2004.01039.x Schwarz Frank, 2006, Schweiz Monatsschr Zahnmed, V116, P1112 Schwarz F, 2006, CLIN ORAL IMPLAN RES, V17, P403, DOI 10.1111/j.1600-0501.2005.01225.x Singer AJ, 1999, NEW ENGL J MED, V341, P738 Taguchi Y, 2005, BIOMATERIALS, V26, P6158, DOI 10.1016/j.biomaterials.2005.03.023 TAMADA JA, 1993, P NATL ACAD SCI USA, V90, P552, DOI 10.1073/pnas.90.2.552 Tatakis DN, 1999, PERIODONTOL 2000, V19, P59, DOI 10.1111/j.1600-0757.1999.tb00147.x Zhao SJ, 2000, J CRANIO MAXILL SURG, V28, P116, DOI 10.1054/jcms.2000.0127 Siar, Chong Huat Toh, Chooi Gait Romanos, George Ng, Kok Han University of MalayaRD 40-02-03-0002 This research was supported by the University of Malaya Grant R&D 40-02-03-0002. Wiley-blackwell publishing, inc Malden
    Uncontrolled Keywords: Animal studies collagenous histomorphometry macaque model noncollagenous guided bone regeneration foreign-body reaction barrier membranes histological-evaluation rat tissue cells biodegradation fibroblasts expression
    Subjects: R Medicine > RK Dentistry
    Divisions: Faculty of Dentistry > Dept of Oral Pathology & Oral Medicine & Periodontology
    Depositing User: Ms Nursyafiqah Abd Malek
    Date Deposited: 10 Jan 2013 08:14
    Last Modified: 10 Jan 2013 08:14
    URI: http://eprints.um.edu.my/id/eprint/4338

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