A preliminary study of healing of superpulsed carbon dioxide laser incisions in the hard palate of monkeys

Romanos, G.; Siar, C.H.; Ng, K.; Toh, C.G. (1999) A preliminary study of healing of superpulsed carbon dioxide laser incisions in the hard palate of monkeys. Lasers in Surgery and Medicine, 24 (5). pp. 368-374. ISSN 0196-8092

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    Abstract

    Background and Objective: Prior studies of laser wound healing using different animal models have shown a delay ed tissue response after carbon dioxide (CO,) laser application. This article reports on the preliminary findings of healing of superpulsed CO2 laser and scalpel incisions in the hard palate of monkeys. Study design/Materials and Methods: Twelve parallel incisions using a superpulsed, continuous wave CO, laser and a scalpel were performed in the hard palate of each of two adult monkeys at 3, 7, and 14 days time schedules. Power levels of 2.0, 4.0, and 6.0 Watts were used for the laser incisions. Wounds were harvested, fixed in 10 formalsaline for at least 48 hours and processed routinely. Each specimen was embedded in paraffin wax at 90 degrees to the surface epithelium and 5 mu m thick sections prepared for staining with haematoxylin and eosin, Periodic acid Schiff and Masson-trichrome at a step-serial interval of 100 mu m. Sections were evaluated independently. Results: According to the clinical findings we showed a wound closure in all of the wounds (laser and scalpel incisions) at 3, 7, and 14 days of healing. Histologically, we showed that laser incisions at three and seven days demonstrated an increased, power setting-dependent tissue necrosis and marked inflammatory response with minimal organization compared to scalpel incisions. At 14 days both types of incisions exhibited complete wound healing of the epithelium and connective tissue. Discussion and Conclusions: According to these preliminary results, superpulsed CO, laser tends to produce more pronounced changes (due to tissue thermal damage) with corresponding greater inflammatory reaction and delay in tissue organization only initially. (C) 1999 Wiley-Liss, Inc.

    Item Type: Article
    Creators:
    1. Romanos, G.
    2. Siar, C.H.
    3. Ng, K.
    4. Toh, C.G.
    Journal or Publication Title: Lasers in Surgery and Medicine
    Additional Information: ISI Document Delivery No.: 210QW Times Cited: 11 Cited Reference Count: 13 Cited References: FISHER SE, 1983, ARCH ORAL BIOL, V28, P287, DOI 10.1016/0003-9969(83)90069-9 FITZPATRICK RE, 1991, J DERMATOL SURG ONC, V17, P340 HOOKS TW, 1980, J ORAL SURG, V49, P263 HUKKI J, 1989, LASER SURG MED, V9, P589, DOI 10.1002/lsm.1900090609 LUOMANEN M, 1987, J ORAL PATHOL MED, V16, P322, DOI 10.1111/j.1600-0714.1987.tb00702.x MORENO RA, 1984, J DERMATOL SURG ONC, V10, P863 POGREL MA, 1990, ORAL SURG ORAL MED O, V69, P269, DOI 10.1016/0030-4220(90)90285-Z ROMANOS GE, 1995, LASER SURG MED, V16, P368, DOI 10.1002/lsm.1900160408 Schaffer Christopher J., 1997, Wound Repair and Regeneration, V5, P52, DOI 10.1046/j.1524-475X.1997.50112.x Schmidt BL, 1997, ORAL SURG ORAL MED O, V83, P206, DOI 10.1016/S1079-2104(97)90007-X WILDERSMITH P, 1995, ORAL SURG ORAL MED O, V79, P685, DOI 10.1016/S1079-2104(05)80300-2 WINTER GD, 1962, NATURE, V193, P293, DOI 10.1038/193293a0 YU W, 1994, LASER SURG MED, V15, P281, DOI 10.1002/lsm.1900150308 Romanos, G Siar, CH Ng, K Toh, CG Wiley-liss New york
    Uncontrolled Keywords: CO2 laser monkey superpulse oral tissue wound healing nd-yag laser rat skin scalpel co2-laser excision wounds mode
    Subjects: R Medicine > RK Dentistry
    Divisions: Faculty of Dentistry > Dept of Oral Pathology & Oral Medicine & Periodontology
    Depositing User: Ms Nursyafiqah Abd Malek
    Date Deposited: 05 Dec 2012 09:56
    Last Modified: 05 Dec 2012 09:56
    URI: http://eprints.um.edu.my/id/eprint/4312

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