Al-Mekhlafi, Hesham M. and Madkhali, Aymen M. and Abdulhaq, Ahmed A. and Atroosh, Wahib Mohsen and Ghzwani, Ahmad Hassn and Zain, Khalid Ammash and Ghailan, Khalid Y. and Hamali, Hassan A. and Mobarki, Abdullah A. and Alharazi, Talal H. and Eisa, Zaki M. and Lau, Yee-Ling (2022) Polymorphism analysis of pfmdr1 gene in Plasmodium falciparum isolates 11 years post-adoption of artemisinin-based combination therapy in Saudi Arabia. Scientific Reports, 12 (1). ISSN 2045-2322, DOI https://doi.org/10.1038/s41598-021-04450-x.
Full text not available from this repository.Abstract
A total of 227 Plasmodium falciparum isolates from Jazan region, southwestern Saudi Arabia were amplified for the P. falciparum multi-drug resistance 1 (pfmdr1) gene to detect point mutations 11 years after the introduction of artemisinin-based combination therapy (ACT) in Saudi Arabia. The pfmdr1 86Y mutation was found in 11.5% (26/227) of the isolates while the N86 wild allele was detected in 88.5%. Moreover, 184F point mutations dominated (86.3%) the instances of pfmdr1 polymorphism while no mutation was observed at codons 1034, 1042 and 1246. Three pfmdr1 haplotypes were identified, NFSND (74.9%), NYSND (13.7%) and YFSND (11.4%). Associations of the prevalence of 86Y mutation and YFSND haplotype with participants' nationality, residency and parasitaemia level were found to be significant (P < 0.05). The findings revealed significant decline in the prevalence of the pfmdr1 86Y mutation in P. falciparum isolates from Jazan region over a decade after the implementation of ACT treatment. Moreover, the high prevalence of the NFSND haplotype might be indicative of the potential emergence of CQ-sensitive but artemether-lumefantrine-resistant P. falciparum strains since the adoption of ACT. Therefore, continuous monitoring of the molecular markers of antimalarial drug resistance in Jazan region is highly recommended.
| Item Type: | Article |
|---|---|
| Funders: | Research Groups funding programme of the Deanship of Scientific Research, Jazan University (Vector-Borne Diseases Research Group) [Grant No: RG-2-1] |
| Uncontrolled Keywords: | Plasmodium falciparum; Jazan region; Antimalarial drug resistance; Mutations |
| Subjects: | R Medicine > RM Therapeutics. Pharmacology |
| Divisions: | Faculty of Medicine > Parasitology Deparment |
| Depositing User: | Ms. Juhaida Abd Rahim |
| Date Deposited: | 05 Sep 2023 08:18 |
| Last Modified: | 27 Nov 2024 01:59 |
| URI: | http://eprints.um.edu.my/id/eprint/43074 |
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