Distribution of basement membrane type IV collagen alpha chains in ameloblastoma: an immunofluorescence study

Nakano, K.; Siar, C.H.; Nagai, N.; Naito, I.; Sado, Y.; Nagatsuka, H.; Hoh, C.; Kurada, K.; Tsujigiwa, H.; Gunduz, M. (2002) Distribution of basement membrane type IV collagen alpha chains in ameloblastoma: an immunofluorescence study. Journal of Oral Pathology & Medicine, 31 (8). pp. 494-499. ISSN 0904-2512

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    Abstract

    Background: Type IV collagen, a heterotrimeric molecule that exists in six genetically distinct forms, alpha1(IV)-alpha6(IV) is a major structural component of basement membrane (BM) and acts as a scaffold for other BM constituents. Methods: Indirect immunofluorescence using alpha chain-specific monoclonal antibodies was employed to clarify basement membrane (BM) collagen IV distribution in two ameloblastoma, and for comparison, on oral mucosa and tooth germ. Results: Ameloblastoma BM expressed five of six genetically distinct forms of collagen IV: alpha1(IV), alpha2(IV), alpha5(IV) and alpha6(IV) chains occurred as intense linear stainings without disruption around neoplastic epithelium, and this expression pattern was fundamentally similar to oral mucosa BM; alpha4(IV) expression was rare and occurred around nests of primitive tumor cells or potentially invasive sites. The tooth germ demonstrated a stage- and position-specific collagen IV distribution: the inner enamel epithelium BM expressed alpha1(IV), alpha2(IV), and alpha4(IV) except in the cuspal predentine region; and the outer enamel epithelium BM expressed alpha1(V), alpha2(IV), alpha5(IV), and alpha6(IV) chains. Conclusions: Results suggest that collagen IV alpha chain distribution in ameloblastoma BM plays an important role in tumor cytodifferentiation and progression.

    Item Type: Article
    Creators:
    1. Nakano, K.
    2. Siar, C.H.
    3. Nagai, N.
    4. Naito, I.
    5. Sado, Y.
    6. Nagatsuka, H.
    7. Hoh, C.
    8. Kurada, K.
    9. Tsujigiwa, H.
    10. Gunduz, M.
    Journal or Publication Title: Journal of Oral Pathology & Medicine
    Additional Information: ISI Document Delivery No.: 594KC Times Cited: 14 Cited Reference Count: 22 Cited References: Fleischmajer R, 1997, J INVEST DERMATOL, V109, P527, DOI 10.1111/1523-1747.ep12336696 HEIKINHEIMO K, 1991, VIRCHOWS ARCH B, V61, P101 HEIKINHEIMO K, 1995, J DENT RES, V74, P1226 HUDSON BG, 1993, J BIOL CHEM, V268, P26033 Kagawa M, 1997, NEPHROL DIAL TRANSPL, V12, P1238, DOI 10.1093/ndt/12.6.1238 Kalluri R, 2000, J BIOL CHEM, V275, P12719, DOI 10.1074/jbc.275.17.12719 KRAMER IRH, 1992, INT HISTOLOGICAL CLA Melrose RJ, 1999, SEMIN DIAGN PATHOL, V16, P271 Nagai N, 2001, INT J DEV BIOL, V45, P827 NINOMIYA Y, 1995, J CELL BIOL, V1, P219 PHILIPSEN HP, 1992, INT J ORAL MAX SURG, V21, P352, DOI 10.1016/S0901-5027(05)80761-1 SADO Y, 1995, HISTOCHEM CELL BIOL, V104, P267, DOI 10.1007/BF01464322 SAKU T, 1992, J ORAL PATHOL MED, V21, P113, DOI 10.1111/j.1600-0714.1992.tb00993.x Salo T, 1999, J ORAL PATHOL MED, V28, P337 SAUK JJ, 1985, J ORAL PATHOL MED, V14, P307, DOI 10.1111/j.1600-0714.1985.tb00498.x SAUS J, 1988, J BIOL CHEM, V263, P13374 Takata T, 2000, VIRCHOWS ARCH, V436, P319, DOI 10.1007/s004280050453 Takata T, 2000, VIRCHOWS ARCH, V436, P324, DOI 10.1007/s004280050454 THESLEFF I, 1984, J ORAL PATHOL MED, V13, P85, DOI 10.1111/j.1600-0714.1984.tb01404.x THESLEFF I, 1995, INT J DEV BIOL, V39, P35 THESLEFF I, 1981, DEV BIOL, V81, P182, DOI 10.1016/0012-1606(81)90361-4 YURCHENCO PD, 1994, CURR OPIN CELL BIOL, V6, P674, DOI 10.1016/0955-0674(94)90093-0 Nakano, K Siar, CH Nagai, N Naito, I Sado, Y Nagatsuka, H Hoh, C Kurada, K Tsujigiwa, H Gunduz, M Blackwell munksgaard Copenhagen
    Uncontrolled Keywords: Ameloblastoma collagen IV profile indirect immunofluorescence defined monoclonal-antibodies human fetal teeth odontogenic-tumors immunohistochemical demonstration basal lamina expression proteins benign tooth
    Subjects: R Medicine > RK Dentistry
    Divisions: Faculty of Dentistry > Dept of Oral Pathology & Oral Medicine & Periodontology
    Depositing User: Ms Nursyafiqah Abd Malek
    Date Deposited: 09 Jan 2013 09:11
    Last Modified: 09 Jan 2013 09:11
    URI: http://eprints.um.edu.my/id/eprint/4296

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