Distribution of basement membrane type IV collagen alpha chains in ameloblastoma: an immunofluorescence study

Nakano, K. and Siar, C.H. and Nagai, N. and Naito, I. and Sado, Y. and Nagatsuka, H. and Hoh, C. and Kurada, K. and Tsujigiwa, H. and Gunduz, M. (2002) Distribution of basement membrane type IV collagen alpha chains in ameloblastoma: an immunofluorescence study. Journal of Oral Pathology & Medicine, 31 (8). pp. 494-499. ISSN 0904-2512

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Abstract

Background: Type IV collagen, a heterotrimeric molecule that exists in six genetically distinct forms, alpha1(IV)-alpha6(IV) is a major structural component of basement membrane (BM) and acts as a scaffold for other BM constituents. Methods: Indirect immunofluorescence using alpha chain-specific monoclonal antibodies was employed to clarify basement membrane (BM) collagen IV distribution in two ameloblastoma, and for comparison, on oral mucosa and tooth germ. Results: Ameloblastoma BM expressed five of six genetically distinct forms of collagen IV: alpha1(IV), alpha2(IV), alpha5(IV) and alpha6(IV) chains occurred as intense linear stainings without disruption around neoplastic epithelium, and this expression pattern was fundamentally similar to oral mucosa BM; alpha4(IV) expression was rare and occurred around nests of primitive tumor cells or potentially invasive sites. The tooth germ demonstrated a stage- and position-specific collagen IV distribution: the inner enamel epithelium BM expressed alpha1(IV), alpha2(IV), and alpha4(IV) except in the cuspal predentine region; and the outer enamel epithelium BM expressed alpha1(V), alpha2(IV), alpha5(IV), and alpha6(IV) chains. Conclusions: Results suggest that collagen IV alpha chain distribution in ameloblastoma BM plays an important role in tumor cytodifferentiation and progression.

Item Type: Article
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Uncontrolled Keywords: Ameloblastoma collagen IV profile indirect immunofluorescence defined monoclonal-antibodies human fetal teeth odontogenic-tumors immunohistochemical demonstration basal lamina expression proteins benign tooth
Subjects: R Medicine > RK Dentistry
Divisions: Faculty of Dentistry > Dept of Oral Pathology & Oral Medicine & Periodontology
Depositing User: Ms Nursyafiqah Abd Malek
Date Deposited: 09 Jan 2013 01:11
Last Modified: 09 Jan 2013 01:11
URI: http://eprints.um.edu.my/id/eprint/4296

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