Distribution of basement membrane type IV collagen alpha chains in ameloblastoma: an immunofluorescence study

Nakano, K.; Siar, C.H.; Nagai, N.; Naito, I.; Sado, Y.; Nagatsuka, H.; Hoh, C.; Kurada, K.; Tsujigiwa, H.; Gunduz, M. (2002) Distribution of basement membrane type IV collagen alpha chains in ameloblastoma: an immunofluorescence study. Journal of Oral Pathology & Medicine, 31 (8). pp. 494-499. ISSN 0904-2512

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    Background: Type IV collagen, a heterotrimeric molecule that exists in six genetically distinct forms, alpha1(IV)-alpha6(IV) is a major structural component of basement membrane (BM) and acts as a scaffold for other BM constituents. Methods: Indirect immunofluorescence using alpha chain-specific monoclonal antibodies was employed to clarify basement membrane (BM) collagen IV distribution in two ameloblastoma, and for comparison, on oral mucosa and tooth germ. Results: Ameloblastoma BM expressed five of six genetically distinct forms of collagen IV: alpha1(IV), alpha2(IV), alpha5(IV) and alpha6(IV) chains occurred as intense linear stainings without disruption around neoplastic epithelium, and this expression pattern was fundamentally similar to oral mucosa BM; alpha4(IV) expression was rare and occurred around nests of primitive tumor cells or potentially invasive sites. The tooth germ demonstrated a stage- and position-specific collagen IV distribution: the inner enamel epithelium BM expressed alpha1(IV), alpha2(IV), and alpha4(IV) except in the cuspal predentine region; and the outer enamel epithelium BM expressed alpha1(V), alpha2(IV), alpha5(IV), and alpha6(IV) chains. Conclusions: Results suggest that collagen IV alpha chain distribution in ameloblastoma BM plays an important role in tumor cytodifferentiation and progression.

    Item Type: Article
    1. Nakano, K.
    2. Siar, C.H.
    3. Nagai, N.
    4. Naito, I.
    5. Sado, Y.
    6. Nagatsuka, H.
    7. Hoh, C.
    8. Kurada, K.
    9. Tsujigiwa, H.
    10. Gunduz, M.
    Journal or Publication Title: Journal of Oral Pathology & Medicine
    Additional Information: ISI Document Delivery No.: 594KC Times Cited: 14 Cited Reference Count: 22 Cited References: Fleischmajer R, 1997, J INVEST DERMATOL, V109, P527, DOI 10.1111/1523-1747.ep12336696 HEIKINHEIMO K, 1991, VIRCHOWS ARCH B, V61, P101 HEIKINHEIMO K, 1995, J DENT RES, V74, P1226 HUDSON BG, 1993, J BIOL CHEM, V268, P26033 Kagawa M, 1997, NEPHROL DIAL TRANSPL, V12, P1238, DOI 10.1093/ndt/12.6.1238 Kalluri R, 2000, J BIOL CHEM, V275, P12719, DOI 10.1074/jbc.275.17.12719 KRAMER IRH, 1992, INT HISTOLOGICAL CLA Melrose RJ, 1999, SEMIN DIAGN PATHOL, V16, P271 Nagai N, 2001, INT J DEV BIOL, V45, P827 NINOMIYA Y, 1995, J CELL BIOL, V1, P219 PHILIPSEN HP, 1992, INT J ORAL MAX SURG, V21, P352, DOI 10.1016/S0901-5027(05)80761-1 SADO Y, 1995, HISTOCHEM CELL BIOL, V104, P267, DOI 10.1007/BF01464322 SAKU T, 1992, J ORAL PATHOL MED, V21, P113, DOI 10.1111/j.1600-0714.1992.tb00993.x Salo T, 1999, J ORAL PATHOL MED, V28, P337 SAUK JJ, 1985, J ORAL PATHOL MED, V14, P307, DOI 10.1111/j.1600-0714.1985.tb00498.x SAUS J, 1988, J BIOL CHEM, V263, P13374 Takata T, 2000, VIRCHOWS ARCH, V436, P319, DOI 10.1007/s004280050453 Takata T, 2000, VIRCHOWS ARCH, V436, P324, DOI 10.1007/s004280050454 THESLEFF I, 1984, J ORAL PATHOL MED, V13, P85, DOI 10.1111/j.1600-0714.1984.tb01404.x THESLEFF I, 1995, INT J DEV BIOL, V39, P35 THESLEFF I, 1981, DEV BIOL, V81, P182, DOI 10.1016/0012-1606(81)90361-4 YURCHENCO PD, 1994, CURR OPIN CELL BIOL, V6, P674, DOI 10.1016/0955-0674(94)90093-0 Nakano, K Siar, CH Nagai, N Naito, I Sado, Y Nagatsuka, H Hoh, C Kurada, K Tsujigiwa, H Gunduz, M Blackwell munksgaard Copenhagen
    Uncontrolled Keywords: Ameloblastoma collagen IV profile indirect immunofluorescence defined monoclonal-antibodies human fetal teeth odontogenic-tumors immunohistochemical demonstration basal lamina expression proteins benign tooth
    Subjects: R Medicine > RK Dentistry
    Divisions: Faculty of Dentistry > Dept of Oral Pathology & Oral Medicine & Periodontology
    Depositing User: Ms Nursyafiqah Abd Malek
    Date Deposited: 09 Jan 2013 09:11
    Last Modified: 09 Jan 2013 09:11
    URI: http://eprints.um.edu.my/id/eprint/4296

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