Differential expression of collagen IV alpha 1 to alpha 6 chains in basement membranes of benign and malignant odontogenic tumors

Nagatsuka, H. and Siar, C.H. and Nakano, K. and Tsujigiwa, H. and Gunduz, M. and Choufuku, H. and Lee, Y.J. and Naito, I. and Sado, Y. and Nagai, N. (2002) Differential expression of collagen IV alpha 1 to alpha 6 chains in basement membranes of benign and malignant odontogenic tumors. Virchows Archiv, 441 (4). pp. 392-399. ISSN 0945-6317


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Type IV collagen, the major component of basement membrane (BM), demonstrates a stage- and position-specific distribution of its isoforms during tooth development. To determine its localization in BM of odontogenic neoplasms, immunohistochemistry using six anti-a(IV) chain-specific monoclonal antibodies was performed. Results disclosed that BM demonstrated an irregular a(IV) chain profile in malignant odontogenic tumors as compared to benign odontogenic neoplasms. No alpha3(IV) chains were detected. Expression of alpha1(IV)/alpha2(IV) and alpha5(IV)/alpha6(IV) chains was stronger in desmoplastic (n=3) than in ordinary (n=5) ameloblastomas. The adenomatoid odontogenic tumor (n=2) distinctly expressed these chains in BM of cribriform areas and hyaline materials (which was also alpha4(IV)-positive), but weakly around epithelial whorls/rosettes/nests and mineralized foci. These five chains also stained BM and tumor cells of ameloblastic fibroma (n=3) and ameloblastic fibro-odontosarcoma (n=1), but not the inductive hard tissues. Ameloblastic carcinoma (n=2) showed specific alpha1(IV)/alpha2(IV) chain loss, while primary intraosseous carcinoma (n=1) demonstrated a discontinuous alpha1(IV)/alpha2(IV) and alpha5(IV)/alpha6(IV) staining pattern. The present results suggest that modification and remodeling of BM collagen IValpha chains occur during odontogenic neoplasms' progression.

Item Type: Article
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Uncontrolled Keywords: Type IV collagen basement membrane immunohistochemistry odontogenic tumors human fetal teeth immunohistochemical demonstration enamel proteins basal lamina carcinoma ameloblastomas localization breast tooth
Subjects: R Medicine > RK Dentistry
Divisions: Faculty of Dentistry > Dept of Oral Pathology & Oral Medicine & Periodontology
Depositing User: Ms Nursyafiqah Abd Malek
Date Deposited: 09 Jan 2013 02:40
Last Modified: 09 Jan 2013 02:40
URI: http://eprints.um.edu.my/id/eprint/4293

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