Localization of type IV membrane during collagen alpha 1 to alpha 6 chains in basement mouse molar germ development

Nagai, N. and Nakano, K. and Sado, Y. and Naito, I. and Gunduz, M. and Tsujigiwa, H. and Nagatsuka, H. and Ninomiya, Y. and Siar, C.H. (2001) Localization of type IV membrane during collagen alpha 1 to alpha 6 chains in basement mouse molar germ development. International Journal of Developmental Biology, 45 (7). pp. 827-831. ISSN 0214-6282,

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Abstract

The dental basement membrane (BM) putatively mediates epithelial-mesenchymal interactions during tooth morphogenesis and cytodifferentiation. Type IV collagen a chains, a major network-forming protein of the dental BM, was studied and results disclosed distinct expression patterns at different stages of mouse molar germ development. At the dental placode and bud stage, the BM of the oral epithelium expressed alpha1, alpha2, alpha5 and alpha6 chains while the gubernaculum dentis, in addition to the above four chains, also expressed alpha4 chain. An asymmetrical expression for alpha4, alpha5 and alpha6 chains was observed at the bud stage. At the early bell stage, the BM associated with the inner enamel epithelium (IEE) of molar germ expressed alpha1, alpha2 and alpha4 chains while the BM of the outer enamel epithelium (OEE) expressed only alpha1 and alpha2 chains. With the onset of dentinogenesis, the collagen alpha chain profile of the IEE BM gradually disappeared. However from the early to late bell stage, the gubernaculum dentis consistently expressed alpha1, alpha2, alpha5 and alpha6 chains resembling fetal oral mucosa. These findings suggest that stage- and position-specific distribution of type IV collagen alpha subunits occur during molar germ development and that these changes are essential for molar morphogenesis and cytodifferentiation.

Item Type:	Article
Funders:	UNSPECIFIED
Additional Information:	ISI Document Delivery No.: 493NK Times Cited: 12 Cited Reference Count: 22 Cited References: Aberg T, 1997, DEV DYNAM, V210, P383, DOI 10.1002/(SICI)1097-0177(199712)210:4<383::AID-AJA3>3.0.CO;2-C Fleischmajer R, 1997, J INVEST DERMATOL, V109, P527, DOI 10.1111/1523-1747.ep12336696 HEIKINHEIMO K, 1995, J DENT RES, V74, P1226 HUDSON BG, 1993, J BIOL CHEM, V268, P26033 Kagawa M, 1997, NEPHROL DIAL TRANSPL, V12, P1238, DOI 10.1093/ndt/12.6.1238 KUBLER MD, 1988, ROUX ARCH DEV BIOL, V197, P212, DOI 10.1007/BF02439428 LESOT H, 1981, DEV BIOL, V82, P371, DOI 10.1016/0012-1606(81)90460-7 Miner JH, 1996, J CELL BIOL, V135, P1403, DOI 10.1083/jcb.135.5.1403 NAGAI N, 1995, J HARD TISSUE BIOL, V4, P15 NINOMIYA Y, 1995, J CELL BIOL, V130, P1219, DOI 10.1083/jcb.130.5.1219 PARALKAR VM, 1991, DEV BIOL, V143, P303, DOI 10.1016/0012-1606(91)90081-D Petitclerc E, 2000, J BIOL CHEM, V275, P8051, DOI 10.1074/jbc.275.11.8051 RUCH JV, 1983, J BIOL BUCCALE, V11, P173 RUCH JV, 1987, CELL BIOL REV, V14, P1 SADO Y, 1995, HISTOCHEM CELL BIOL, V104, P267, DOI 10.1007/BF01464322 SAUS J, 1988, J BIOL CHEM, V263, P13374 Seki T, 1998, HISTOCHEM CELL BIOL, V110, P359, DOI 10.1007/s004180050296 THESLEFF I, 1995, INT J DEV BIOL, V39, P35 THESLEFF I, 1981, DEV BIOL, V81, P182, DOI 10.1016/0012-1606(81)90361-4 Yoshiba K, 1998, DEV DYNAM, V211, P164, DOI 10.1002/(SICI)1097-0177(199802)211:2<164::AID-AJA5>3.0.CO;2-F Yoshiba K, 2000, INT J DEV BIOL, V44, P337 YURCHENCO PD, 1994, CURR OPIN CELL BIOL, V6, P674, DOI 10.1016/0955-0674(94)90093-0 Nagai, N Nakano, K Sado, Y Naito, I Gunduz, M Tsujigiwa, H Nagatsuka, H Ninomiya, Y Siar, CH U b c press Bilbao
Uncontrolled Keywords:	Type IV collagen alpha chains molar germ development basement membrane immunofluorescence localization defined monoclonal-antibodies differential expression laminin-5 subunits tooth development basal lamina alpha-5(iv) fibronectin alpha-3(iv) matrix teeth
Subjects:	R Medicine > RK Dentistry
Divisions:	Faculty of Dentistry > Dept of Oral Pathology & Oral Medicine & Periodontology
Depositing User:	Ms Nursyafiqah Abd Malek
Date Deposited:	09 Jan 2013 02:37
Last Modified:	09 Jan 2013 02:37
URI:	http://eprints.um.edu.my/id/eprint/4289

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