Immunohistochemical examination of cytological differentiation in osteosarcomas

Kawakami, T.; Shimizu, T.; Kimura, A.; Hasegawa, H.; Siar, C.H.; Ng, K. H.; Nagatsuka, H.; Nagai, N.; Kanda, H. (2005) Immunohistochemical examination of cytological differentiation in osteosarcomas. European Journal of Medical Research, 10 (11). pp. 475-479. ISSN 0949-2321

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      Abstract

      In this immunohistochemical examination, the expression of Runx2, Notch1, Delta and Osteopontin peptides were detected in neoplastic cells in 10 Japanese cases of osteosarconia. Immunohistochemically Runx2 peptide expression appeared in the cytoplasm of almost all neoplastic cells of the 10 cases examined. However, Notch1 peptide expression appeared in the cytoplasm of neoplastic cells in the localized and comparatively well-differentiated area of osteosarcorna, which osteoblastic and chondroblastic containing osteoid and/or chondroid tissues. No expression of Notch1 peptide was detected in the fibroblastic and poorly differentiated areas. Delta peptide appearance was nearly the same pattern of Notch1 peptide. Expression of Osteopontin peptide appeared in almost all cells and the strength expression was shown in the area of comparatively well-differentiated tissues. Therefore, these results suggest that Runx2, Notch1, and Delta peptides are closely related to cytological differentiation or acquisition of tissue specific characteristics in neoplastic cells in osreosarcomas.

      Item Type: Article
      Creators:
      1. Kawakami, T.
      2. Shimizu, T.
      3. Kimura, A.
      4. Hasegawa, H.
      5. Siar, C.H.
      6. Ng, K. H.
      7. Nagatsuka, H.
      8. Nagai, N.
      9. Kanda, H.
      Journal or Publication Title: European Journal of Medical Research
      Additional Information: ISI Document Delivery No.: 990MT Times Cited: 6 Cited Reference Count: 11 Cited References: Andela VB, 2005, J CLIN PATHOL, V58, P328, DOI 10.1136/jcp.2004.017640 Blaumueller CM, 1997, CELL, V90, P281, DOI 10.1016/S0092-8674(00)80336-0 GORSKI JP, 1990, J BIOL CHEM, V265, P14956 Kawakami T, 2004, EUR J MED RES, V9, P533 Nobta M, 2005, J BIOL CHEM, V280, P15842, DOI 10.1074/jbc.M412891200 Qi HL, 1999, SCIENCE, V283, P91, DOI 10.1126/science.283.5398.91 Schnabel M, 2002, INT J MOL MED, V9, P229 Tezuka K, 2002, J BONE MINER RES, V17, P231, DOI 10.1359/jbmr.2002.17.2.231 Thomas DM, 2004, J CELL BIOL, V167, P925, DOI 10.1083/jcb.200409187 Watanabe N, 2003, J BONE MINER METAB, V21, P344, DOI 10.1007/s00774-003-0428-4 ZAGOURAS P, 1995, P NATL ACAD SCI USA, V92, P6414, DOI 10.1073/pnas.92.14.6414 Kawakami, T Shimizu, T Kimura, A Hasegawa, H Siar, CH Ng, KH Nagatsuka, H Nagai, N Kanda, H I holzapfel verlag gmbh Munich
      Uncontrolled Keywords: Osteosarcoma Notch1 Runx2 Delta osteopontin cyrological nature morphogenesis differentiation regulation factor immunohistochemistry osteoblastic cells notch expression bone
      Subjects: R Medicine > RK Dentistry
      Divisions: Faculty of Dentistry > Dept of Oral Pathology & Oral Medicine & Periodontology
      Depositing User: Ms Nursyafiqah Abd Malek
      Date Deposited: 08 Jan 2013 11:57
      Last Modified: 08 Jan 2013 11:57
      URI: http://eprints.um.edu.my/id/eprint/4281

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