Wingless-type Protein-1 (Wnt-1) expression in primary conventional and unicystic ameloblastomas and their recurrent tumors
Chuah, K.S.; Siar, C.H.; Nakano, K.; Nagatsuke, H.; Khoo, S.P.; Ng, K.H.; Kawakami, T. (2009) Wingless-type Protein-1 (Wnt-1) expression in primary conventional and unicystic ameloblastomas and their recurrent tumors. Journal of Hard Tissue Biology, 18 (2). pp. 63-70. ISSN 1341-7649
Wingless-type protein (Wnt) is a family of 19 secreted glycoproteins that function as signaling transducers for cell-cell interaction, cell growth and differentiation. Wnt-1, a highly transforming member, has been implicated in tumorigenesis. The aim of this study was to elucidate the role of this cell signaling molecule in the development and progression of primary ameloblastoma and their recurrent tumors. Wnt-1 expression patterns were examined immunohistochemically in 22 primary and 14 recurrent ameloblastomas. These collectively consisted of the following subtypes: conventional (CA) (n=22), desmoplastic (DA) (n=2) and unicystic (UA) (n=12) ameloblastoma. Results demonstrated that CA (n=20/22; 90.9), DA (n=2/2; 100) and UA (11/12; 91.7) showed high Wnt-1 expression percentages. Strong staining intensity for Wnt-1 was observed more frequently in primary CA (n=10/13; 76.9) than in their recurrent counterparts (n=2/9; 22.2) (p<0.05). Conversely, in UA, recurrent tumors (n=3/5; 60.0) tend to stain strongly for Wnt-1 more frequently than their primary lesions (n=3/7; 42.9) (p>0.05). Keratinizing cells in areas of squamous metaplasia also expressed Wnt-1 more intensely compared to their surrounding polyhedral stellate reticulum-like cells. Tumor islands containing granular cells were also Wnt-1 positive. Present findings confirmed that the Wnt signaling pathway is activated in ameloblastoma. Strong Wnt-1 expression in primary conventional and recurrent unicystic ameloblastoma suggests that Wnt-1 plays a more critical role in these subtypes. Positive expression of Wnt-1 in keratinizing ameloblastomatous tumor epithelium and granular cells complies with the anti-apoptotic properties of Wnt-1. Negative reactivity for Wnt-1 in 3 cases of ameloblastoma suggests that the development and progression of ameloblastoma may occur independent of this cell signaling molecule.
|Item Type: ||Article|
- Chuah, K.S.
- Siar, C.H.
- Nakano, K.
- Nagatsuke, H.
- Khoo, S.P.
- Ng, K.H.
- Kawakami, T.
|Journal or Publication Title: ||Journal of Hard Tissue Biology|
|Additional Information: ||ISI Document Delivery No.: 589PB Times Cited: 6 Cited Reference Count: 27 Cited References: Behrens J, 1996, NATURE, V382, P638, DOI 10.1038/382638a0 Chen X, 2008, ACTA BIOCH BIOPH SIN, V40, P577, DOI 10.1111/j.1745-7270.2008.00440.x Gardner DG, 2005, WHO CLASSIFICATION T, P296 HAN PP, 2006, J HARD TISUE BIOL, V15, P46, DOI 10.2485/jhtb.15.46 He B, 2004, NEOPLASIA, V6, P7 Huang CL, 2008, EUR J CANCER, V44, P2680, DOI 10.1016/j.ejca.2008.08.004 Inoue M, 2008, J HARD TISSUE BIOL, V17, P23, DOI 10.2485/jhtb.17.23 Khor TO, 2006, INT J COLORECTAL DIS, V21, P291, DOI 10.1007/s00384-005-0002-8 Kumamoto H, 2005, J ORAL PATHOL MED, V34, P401, DOI 10.1111/j.1600-0714.2005.00328.x Leocata P, 2007, J ORAL PATHOL MED, V36, P394, DOI 10.1111/j.1600-0714.2007.00537.x Lo Muzio L, 2002, ANTICANCER RES, V22, P565 Miletich I, 2003, HUM MOL GENET, V12, P69 Nagatsuka H, 2002, VIRCHOWS ARCH, V441, P392, DOI 10.1007/s00428-002-0658-1 Nagatsuka H, 2005, ORAL ONCOL, V41, P542, DOI 10.1016/j.oraloncology.2005.01.004 Nakano K, 2008, J HARD TISSUE BIOL, V17, P79 Nakano K, 2002, J ORAL PATHOL MED, V31, P494, DOI 10.1034/j.1600-0714.2002.00162.x NUSSE R, 1982, CELL, V31, P99, DOI 10.1016/0092-8674(82)90409-3 PLATT KA, 1997, MECH DEVELOP, V62 Sandra F, 2001, HISTOPATHOLOGY, V39, P93, DOI 10.1046/j.1365-2559.2001.01138.x Sarkar L, 1999, MECH DEVELOP, V85, P197, DOI 10.1016/S0925-4773(99)00095-7 SATHI GS, 2007, ORAL PATHOL MED, V36, P609 Tanahashi J, 2008, J ORAL PATHOL MED, V37, P565, DOI 10.1111/j.1600-0714.2008.00645.x Tsujigiwa H, 2005, ORAL ONCOL, V41, P843, DOI 10.1016/j.oraloncology.2005.04.005 Veeck J, 2008, BREAST CANCER RES, V10, DOI 10.1186/bcr2151 Willert K, 2003, NATURE, V423, P448, DOI 10.1038/nature01611 Xiao J, 2007, J HARD TISSUE BIOL, V16, P79, DOI 10.2485/jhtb.16.79 Zhang WM, 2005, ONCOL REP, V13, P1095 Chuah, Kee Seng Siar, Chong Huat Nakano, Keisuke Nagatsuke, Hitoshi Khoo, Suan Phaik Ng, Kok Han Kawakami, Toshiyuki University of MalayaF0170/2007A; Japan Society for the Promotion of Science20592349 This study was funded by the University of Malaya Postgraduate Grant (F0170/2007A). This research was partially supported by Grants-in Aid for Scientific Research C (#20592349) from the Japan Society for the Promotion of Science. Journal hard tissue biology Minato ku|
|Uncontrolled Keywords: ||Ameloblastoma Immunohistochemistry Wnt-1 beta-catenin cell carcinoma collagen cancer chains gene|
|Subjects: ||R Medicine > RK Dentistry|
|Divisions: ||Faculty of Dentistry > Dept of Oral Pathology & Oral Medicine & Periodontology|
|Depositing User: ||Ms Nursyafiqah Abd Malek|
|Date Deposited: ||09 Jan 2013 09:12|
|Last Modified: ||09 Jan 2013 09:12|
Actions (login required)