Identification and characterization of Putative Virulence genes and gene clusters in Aeromonas Hydrophila PPD134/91

Yu, H.B.; Zhang, Y.L.; Lau, Y.L.; Yao, F.; Vilches, S.; Merino, S.; Tomas, J.M.; Howard, S.P.; Leung, K.Y. (2005) Identification and characterization of Putative Virulence genes and gene clusters in Aeromonas Hydrophila PPD134/91. Applied and Environmental Microbiology, 71 (8). pp. 4469-4477. ISSN 0099-2240 (Print) 0099-2240 (Linking)

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    Abstract

    Aeromonas hydrophila is a gram-negative opportunistic pathogen of animals and humans. The pathogenesis of A. hydrophila is multifactorial. Genomic subtraction and markers of genomic islands (GIs) were used to) identify putative virulence genes in A. hydrophila PPD134/91. Two rounds of genomic subtraction led to the identification of 22 unique DNA fragments encoding 19 putative virulence factors and seven new open reading frames, which are commonly present in the eight virulence strains examined. In addition, four GIs were found, including O-antigen, capsule, phage-associated, and type III secretion system (TTSS) gene clusters. These putative virulence genes and gene clusters were positioned on a physical map of A. hydrophila PPD134/91 to determine their genetic organization in this bacterium. Further in vivo study of insertion and deletion mutants showed that the TTSS may be one of the important virulence factors in A. hydrophila pathogenesis. Further. more, deletions of multiple virulence factors such as S-layer, serine protease, and metalloprotease also increased the 50 lethal dose to the same level as the TTSS mutation (about 1 log) in a blue gourami infection model. This observation sheds light on the multifactorial and concerted nature of pathogenicity in A. hydrophila. The large number of putative virulence genes identified in this study will form the basis for further investigation of this emerging pathogen and help to develop effective vaccines, diagnostics, and novel therapeutics.

    Item Type: Article
    Creators:
    1. Yu, H.B.
    2. Zhang, Y.L.
    3. Lau, Y.L.(University of Malaya)
    4. Yao, F.
    5. Vilches, S.
    6. Merino, S.
    7. Tomas, J.M.
    8. Howard, S.P.
    9. Leung, K.Y.
    Journal or Publication Title: Applied and Environmental Microbiology
    Additional Information: Yu, HB Zhang, YL Lau, YL Yao, F Vilches, S Merino, S Tomas, JM Howard, SP Leung, KY
    Uncontrolled Keywords: Aeromonas hydrophila/genetics/metabolism/*pathogenicity Animals Bacterial Proteins/*genetics/metabolism Chromosome Mapping Fish Diseases/microbiology/mortality Gene Deletion Genome, Bacterial Genomic Islands Gram-Negative Bacterial Infections/microbiology/mortality/veterinary Molecular Sequence Data *Multigene Family Nucleic Acid Hybridization/methods Perciformes/*microbiology Sequence Analysis, DNA Virulence Virulence Factors/*genetics/metabolism
    Subjects: R Medicine
    Divisions: Faculty of Medicine
    Depositing User: miss munirah saadom
    Date Deposited: 08 Jan 2013 11:57
    Last Modified: 08 Jan 2013 11:57
    URI: http://eprints.um.edu.my/id/eprint/4253

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