Detection and genetic analysis of group II capsules in aeromonas hydrophila

Zhang, Y.L. and Lau, Y.L. and Arakawa, E. and Leung, K.Y. (2003) Detection and genetic analysis of group II capsules in aeromonas hydrophila. Microbiology, 149 (Pt 4). pp. 1051-1060. ISSN 1350-0872 (Print) 1350-0872 (Linking)

[img] PDF
Zhang-2003-Detection_and_geneti.pdf - Published Version
Restricted to Repository staff only

Download (202kB) | Request a copy

Abstract

The genetic organization and sequences of the group II capsule gene cluster of Aeromonas hydrophila PPD134/91 have been determined previously. The purified capsular polysaccharides can increase the ability of avirulent strain PPD35/85 to survive in naive tilapia serum but have no inhibitory effect on the adhesion of PPD134/91 to carp epithelial cells. In this study, the presence of group II capsules among 33 randomly chosen A. hydrophila strains was examined by electron microscopy and genetic analysis. Ten strains were found to produce group II capsules. A PCR detection system was developed to identify two types of group II capsules (IIA and IIB) based on their genetic organization in the region II gene clusters. Group IIA capsules in the authors' collection of A. hydrophila strains are mainly found in the O:18 and O:34 serogroups, while group IIB capsules are found in the O:21 and O:27 serogroups. The presence of group II capsules in A. hydrophila strongly correlates with the serum and phagocyte survival abilities (seven out of ten strains). The results indicate that the authors' PCR detection system can constitute a reliable assay for the classification of group II capsules in A. hydrophila.

Item Type: Article
Additional Information: Zhang, Y L Lau, Y L Arakawa, E Leung, K Y eng England Reading, England 2003/04/11 05:00 Microbiology. 2003 Apr;149(Pt 4):1051-60.
Uncontrolled Keywords: Aeromonas hydrophila/classification/genetics/*metabolism/*pathogenicity Animals Bacterial Capsules/*classification/*genetics/metabolism Bacterial Proteins/genetics/*metabolism Blood Bactericidal Activity Cloning, Molecular Microscopy, Electron Molecular Sequence Data Multigene Family Open Reading Frames/genetics Phagocytes/microbiology Polymerase Chain Reaction Sequence Analysis, DNA Tilapia
Subjects: R Medicine
Divisions: Faculty of Medicine
Depositing User: miss munirah saadom
Date Deposited: 08 Jan 2013 03:43
Last Modified: 08 Jan 2013 03:43
URI: http://eprints.um.edu.my/id/eprint/4246

Actions (login required)

View Item View Item

Downloads

Downloads per month over past year