Khor, Eng-Soon and Mohd Noor, Suzita and Wong, Pooi-Fong (2022) MiR-107 inhibits the sprouting of intersegmental vessels of zebrafish embryos. Protoplasma, 259 (3). pp. 691-702. ISSN 0033-183X, DOI https://doi.org/10.1007/s00709-021-01695-1.
Full text not available from this repository.Abstract
MicroRNAs (miRNAs) play important roles in various biological processes. Our previous study showed that inhibition of MTOR with rapamycin treatment suppressed human endothelial cell tube formation, concomitant with the down-regulation of miR-107. Similarly, inhibition of Ztor by rapamycin also suppressed vascular development in zebrafish embryos. To gain a better understanding of the role of miR-107 and MTOR in vascular development, the present study sought to validate its function by over-expressing miR-107 in zebrafish embryos via microinjection with mimic miR-107 duplexes. Alkaline phosphatase (ALP) staining was used to visualise blood vessels in the zebrafish embryo, and expressions of Pten, Ztor and Rap1 were investigated by immunoblotting. Over-expression of miR-107 in zebrafish embryos inhibited the sprouting of intersegmental vessels (ISVs) with concomitant down-regulation of phosphorylated Rps6 expression, which confirmed the inhibition of Ztor signalling. As expected, pten, which is the target of miR-107, was down-regulated. Interestingly, Rap1, a small GTPase protein that is involved in intersomitic vessels sprouting during angiogenesis, was also down-regulated when miR-107 was over-expressed. Overall, our findings suggest that miR-107 and Ztor-mediated suppression of vascular development in zebrafish embryo involves Rap1.
| Item Type: | Article |
|---|---|
| Funders: | University of Malaya Research Grant, RG500-13HTM, PG306-2016A |
| Uncontrolled Keywords: | Angiogenesis; miR-107; Zebrafish; Ztor; Pten; Rap1 |
| Subjects: | Q Science > QL Zoology |
| Divisions: | Faculty of Medicine Faculty of Medicine > Biomedical Science Department |
| Depositing User: | Ms. Juhaida Abd Rahim |
| Date Deposited: | 19 Nov 2023 12:29 |
| Last Modified: | 19 Nov 2023 12:29 |
| URI: | http://eprints.um.edu.my/id/eprint/42439 |
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