Loh, Alson and Shaibie, Nur Adania and Chan, Po Qhuan and Leong, Pooi Pooi and Segeran, Sharrada and Tan, Boon Keat and Wong, Siew Tung and Koh, Rhun Yian and Chye, Soi Moi and Voon, Kenny (2022) Pteropine orthoreovirus, PRV7S (Sikamat Virus) demonstrates oncolysis in nasopharyngeal carcinoma cell lines. Frontiers in Bioscience-Landmark, 27 (4). ISSN 2768-6701, DOI https://doi.org/10.31083/j.fbl2704138.
Full text not available from this repository.Abstract
Background: Oncolytic properties had been demonstrated in Mammalian Orthoreovirus (MRV) and Avian Orthorevirus (ARV). Besides MRV and ARV, Pteropine Orthoreovirus (PRV) is also categorized under the genus Orthoreovirus. PRV7S (Sikamat virus) is an orthore-ovirus isolated in Malaysia. Present study aims to investigate the oncolytic effects of PRV7S on ranges of nasopharyngeal carcinoma (NPC) cells through apoptosis in comparison to MRV3. Methods: Non-cancerous nasopharyngeal (NCNP) and NPC cells were infected by PRV7S and MRV3. The effects of PRV7S on the proliferation inhibition and apoptotic activity of NPC cells was examined using MTT assay and flow cytometry. Additionally, western blot assay was performed to analyze the expression of RAS and apoptotic protein. Lastly, qPCR assay was performed to demonstrate that PRV7S and MRV3 replicated in infected-NPC and infected-NCNP cells. Results: The proliferation of NPC cells were significantly inhibited after PRV7S infection in a time dependent manner in comparison to infected-NCPC cells. Flow cytometry analysis showed that PRV7S infection was able to induce apoptosis on NPC cells at 48 hpi. Western blot results showed that upon PRV7S infection, N/H/K RAS protein expression was reduced, whereas caspase-3 protein expression increased in NPC cells. qPCR assay showed higher viral load of PRV7S found in infected-NPC compared to infected-NCNP cells. Conclusions: PRV7S inhibits the proliferation and induces apoptosis of NPC cells similar to MRV3. Therefore, PRV7S is a potential oncolytic virus.
| Item Type: | Article |
|---|---|
| Funders: | Fundamental Research Grant Scheme (FRGS), from the Ministry of Higher Education, Malaysia [Grant No: 2019-1], International Medical University |
| Uncontrolled Keywords: | Pteropine orthoreovirus; Nasopharyngeal carcinoma; Mammalian orthoreovirus; Oncolytic virus; Apoptosis; Bat reovirus |
| Subjects: | Q Science > QD Chemistry Q Science > QH Natural history > QH301 Biology |
| Divisions: | Faculty of Medicine |
| Depositing User: | Ms. Juhaida Abd Rahim |
| Date Deposited: | 12 Oct 2023 08:11 |
| Last Modified: | 12 Oct 2023 08:11 |
| URI: | http://eprints.um.edu.my/id/eprint/42279 |
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