Isolation of Japanese encephalitis virus from mosquitoes collected in Sabak Bernam, Selangor, Malaysia in 1992

Vythilingam, I. and Oda, K. and Chew, T.K. and Mahadevan, S. and Vijayamalar, B. and Morita, K. and Tsuchie, H. and Igarashi, A. (1995) Isolation of Japanese encephalitis virus from mosquitoes collected in Sabak Bernam, Selangor, Malaysia in 1992. Journal of the American Mosquito Control Association, 11 (1). p. 94. ISSN 8756-971X

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Official URL: http://ukpmc.ac.uk/abstract/MED/7616198

Abstract

Detection and isolation of Japanese encephalitis (JE) virus were attempted from female mosquitoes collected in Kampong Pasir Panjang, Sabak Bernam, Selangor, from May to November 1992. A total of 7,400 mosquitoes consisting of 12 species in 148 pools were processed and inoculated into Aedes albopictus clone C6/36 cell cultures. Of these, 26 pools showed the presence of viral antigens in the infected C6/36 cells by specific immunoperoxidase staining using an anti-JE virus polyclonal antibody. Presence of JE virus genome was confirmed in the infected culture fluid for 16 pools by using reverse transcriptase-polymerase chain reaction and JE virus-specific primers. Of these, 3 pools were from Culex tritaeniorhynchus, 4 from Culex vishnui, 3 from Culex bitaeniorhynchus, 2 from Culex sitiens, one from Aedes species, and 3 from Culex species. Isolation of JE virus from Cx. sitiens, Cx. bitaeniorhynchus, and Aedes sp. (Aedes butleri and Ae. albopictus) is reported for the first time in Malaysia.

Item Type: Article
Additional Information: Department of Parasitology, Faculty of Medicine Building, University of Malaya, 50603 Kuala Lumpur, MALAYSIA
Uncontrolled Keywords: Reverse transcriptase polymerase chain reaction; Aedes albopictus; Isolation ; Japanese encephalitis virus ; Vector ; Malaysia ; Culicidae ; Flavivirus ; Flaviviridae ; Virus ; Asia ; Diptera ; Insecta ; Arthropoda ; Invertebrata
Subjects: R Medicine
Divisions: Faculty of Medicine
Depositing User: Miss Nur Jannatul Adnin Ahmad Shafawi
Date Deposited: 07 Jan 2013 03:01
Last Modified: 07 Jan 2013 03:01
URI: http://eprints.um.edu.my/id/eprint/4218

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