Adib, Fatemeh Rastegar and Bagheri, Fatemeh and Sharifi, Ali Mohammad (2022) Osteochondral regeneration in rabbit using xenograft decellularized ECM in combination with different biological products; platelet-rich fibrin, amniotic membrane extract, and mesenchymal stromal cells. Journal of Biomedical Materials Research Part B: Applied Biomaterials, 110 (9). pp. 2089-2099. ISSN 1552-4973, DOI https://doi.org/10.1002/jbm.b.35063.
Full text not available from this repository.Abstract
This study aimed to investigate the regenerative effect of decellularized osteochondral ECM xenograft in combination with various biological products in an osteochondral (OC) defect. OC tissue from the sheep femur were obtained and decellularized. The decellularized ECM (dECM) was combined with either platelet-rich fibrin (PRF), amniotic membrane extract (AME), or rabbit bone marrow-derived mesenchymal stromal cells (rBMSCs). The hybrid dECM-biological products were then utilized for the treatment of rabbit OC critical size defects. The regenerative potential of different groups was compared using; MRI, macroscopic assessment, histopathology, and histomorphometry. All characterizations analysis verified successful decellularization. Three months post-surgery, macroscopic findings indicated that dECM was better compared to controls. Also, dECM in combination with AME, PRF, and rBMSCs showed enhanced OC regeneration compared to only dECM (AME: +100%, PRF: +61%, rBMSCs: +28%). In particular, the dECM+AME group results in the best integration of new cartilage into surrounding cartilage tissue. The histomorphometric evaluations demonstrated enhancement in new cartilage formation and bone tissue (86.5 +/- 5.9% and 90 +/- 7.7%, respectively) for the dECM+AME group compared to other groups. Furthermore, histological results for the dECM+AME elucidated a mature hyaline cartilage tissue that covered the new and symmetrically formed subchondral bone, exhibiting a significantly higher regenerative effect compared to all other treated groups. This finding was also confirmed with MRI images. The current study revealed that in addition to the benefits of dECM alone, its combination with AME indicated to have a superior regenerative effect on OC regeneration. Overall, dECM+AME may be considered a suitable construct for treating knee OC injuries.
| Item Type: | Article |
|---|---|
| Funders: | Iran University of Medical Sciences, 33564 |
| Uncontrolled Keywords: | Amniotic membrane extract; Decellularized ECM; Mesenchymal stromal cells; Osteochondral regeneration; Platelet-rich fibrin |
| Subjects: | R Medicine > RC Internal medicine |
| Divisions: | Faculty of Medicine > Orthopaedic Surgery Department |
| Depositing User: | Ms. Juhaida Abd Rahim |
| Date Deposited: | 19 Nov 2023 14:58 |
| Last Modified: | 19 Nov 2023 14:58 |
| URI: | http://eprints.um.edu.my/id/eprint/42013 |
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