Norpi, Abdin Shakirin Mohamad and Nordin, Muhammad Luqman and Ahmad, Nuraziemah and Katas, Haliza and Ahmad Fuaad, Abdullah Al-Hadi and Sukri, Asif and Marasini, Nirmal and Azmi, Fazren (2022) New modular platform based on multi-adjuvanted amphiphilic chitosan nanoparticles for efficient lipopeptide vaccine delivery against group A streptococcus. Asian Journal of Pharmaceutical Sciences, 17 (3). pp. 435-446. ISSN 1818-0876, DOI https://doi.org/10.1016/j.ajps.2022.04.002.
Full text not available from this repository.Abstract
An effective vaccine against group A streptococcus (GAS) is highly desirable for definitive control of GAS infections. In the present study, two variants of amphiphilic chitosan nanoparticles-based GAS vaccines were developed. The vaccines were primarily composed of encapsulated KLH protein (a source of T helper cell epitopes) and lipidated M-protein derived B cell peptide epitope (lipoJ14) within the amphiphilic structure of nanoparticles. The only difference between them was one of the nanoparticles vaccines received additional surface coating with poly (I:C). The formulated vaccines exhibited nanosized particles within the range of 220-240 nm. Cellular uptake study showed that nanoparticles vaccine without additional poly (I:C) coating has greater uptake by dendritic cells and macrophages compared to nanoparticles vaccine that was functionalized with poly (I:C). Both vaccines were found to be safe in mice and showed negligible cytotoxicity against HEK293 cells. Upon immunization in mice, both nanoparticle vaccines produced high antigen-specific antibodies titres that were regulated by a balanced Th1 and Th2 response compared to physical mixture. These antibodies elicited high opsonic activity against the tested GAS strains. Overall, our data demonstrated that amphiphilic chitosan nanoparticles platform induced a potent immune response even without additional inclusion of poly (I:C). (C) 2022 Shenyang Pharmaceutical University. Published by Elsevier B.V.
| Item Type: | Article |
|---|---|
| Funders: | Universiti Kebangsaan Malaysia (UKM), Malaysia (Grant No: DCP-2017-003/2) |
| Uncontrolled Keywords: | Amphiphilic chitosan nanoparticles; Peptide vaccine; Lipidation; Multi-adjuvanting delivery system; Immunogenicity; Group A streptococcus |
| Subjects: | R Medicine > RM Therapeutics. Pharmacology |
| Divisions: | Faculty of Science > Department of Chemistry |
| Depositing User: | Ms. Juhaida Abd Rahim |
| Date Deposited: | 18 Oct 2023 06:55 |
| Last Modified: | 18 Oct 2023 06:55 |
| URI: | http://eprints.um.edu.my/id/eprint/41998 |
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