Development of a Nested PCR for Environmental Detection of the Pathogenic free-living Amoeba Balamuthia mandrillaris

Ahmad, A.F.; Andrew, P.W.; Kilvington, S. (2011) Development of a Nested PCR for Environmental Detection of the Pathogenic free-living Amoeba Balamuthia mandrillaris. Journal of Eukaryotic Microbiology, 58 (3). pp. 269-271. ISSN 1066-5234

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Abstract

A DNA extraction and nested PCR method for detecting the pathogenic amoeba Balamuthia mandrillaris from the environment was developed. Sixteen of 17 Californian soil samples were positive compared with 0/44 from the United Kingdom. This approach will enable a greater understanding of B. mandrillaris ecology, geographic distribution, and public health risk.

Item Type: Article
Creators:
  1. Ahmad, A.F.(Department of Parasitology, Faculty of Medicine Building, University of Malaya, 50603 Kuala Lumpur, MALAYSIA)
  2. Andrew, P.W.
  3. Kilvington, S.
Journal or Publication Title: Journal of Eukaryotic Microbiology
Additional Information: Ahmad, Arine F. Andrew, Peter W. Kilvington, Simon
Uncontrolled Keywords: Balamuthia; DNA; ecology; molecular; PCR; protozoa; ribosomal; sequence
Subjects: R Medicine
Divisions: Faculty of Medicine
Depositing User: Ms Haslinda Lahuddin
Date Deposited: 13 Dec 2012 10:00
Last Modified: 13 Dec 2012 10:00
URI: http://eprints.um.edu.my/id/eprint/4196

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