Malik, Maqsood Ahmad and Batterjee, Maha G. and Kamli, Majid Rasool and Alzahrani, Khalid Ahmed and Danish, Ekram Y. and Nabi, Arshid (2022) Polyphenol-capped biogenic synthesis of noble metallic silver nanoparticles for antifungal activity against candida auris. Journal of Fungi, 8 (6). ISSN 2309-608X, DOI https://doi.org/10.3390/jof8060639.
Full text not available from this repository.Abstract
In terms of reduced toxicity, the biologically inspired green synthesis of nanoparticles has emerged as a promising alternative to chemically fabricated nanoparticles. The use of a highly stable, biocompatible, and environmentally friendly aqueous extract of Cynara cardunculus as a reducing and capping agent in this study demonstrated the possibility of green manufacturing of silver nanoparticles (CC-AgNPs). UV-visible spectroscopy validated the development of CC-AgNPs, indicating the surface plasmon resonance (SPR) lambda(max) band at 438 nm. The band gap of CC-AgNPs was found to be 2.26 eV. SEM and TEM analysis examined the surface morphology of CC-AgNPs, and micrographs revealed that the nanoparticles were spherical. The crystallinity, crystallite size, and phase purity of as-prepared nanoparticles were confirmed using XRD analysis, and it was confirmed that the CC-AgNPs were a face-centered cubic (fcc) crystalline-structured material. Furthermore, the role of active functional groups involved in the reduction and surface capping of CC-AgNPs was revealed using the Fourier transform infrared (FTIR) spectroscopic technique. CC-AgNPs were mostly spherical and monodispersed, with an average size of 26.89 nm, and were shown to be stable for a longer period without any noticeable change at room temperature. Further, we checked the antifungal mechanism of CC-AgNPs against C. auris MRL6057. The minimum inhibitory concentrations (MIC) and minimum fungicidal concentrations (MFC) were 50.0 mu g/mL and 100.0 mu g/mL respectively. The cell count and viability assay confirmed the fungicidal potential of CC-AgNPs. Further, the analysis showed that CC-AgNPs could induce apoptosis and G2/M phase cell cycle arrest in C. auris MRL6057. Our results also suggest that the CC-AgNPs were responsible for the induction of mitochondrial toxicity. TUNEL assay results revealed that higher concentrations of CC-AgNPs could cause DNA fragmentation. Therefore, the present study suggested that CC-AgNPs hold the capacity for antifungal drug development against C. auris infections.
| Item Type: | Article |
|---|---|
| Funders: | Institutional Fund Projects - Ministry of Education and King Abdulaziz University, Deanship of Scientific Research (DSR), Jeddah, Saudi Arabia [Grant No:IFPHI136-130-2021] |
| Uncontrolled Keywords: | Green synthesis; Polyphenols; Cell cycle; Candida auris |
| Subjects: | Q Science > QR Microbiology |
| Divisions: | Faculty of Science > Department of Chemistry |
| Depositing User: | Ms. Juhaida Abd Rahim |
| Date Deposited: | 17 Oct 2023 01:22 |
| Last Modified: | 17 Oct 2023 01:22 |
| URI: | http://eprints.um.edu.my/id/eprint/41958 |
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