Improvement of biovanillin production with two-stage pH control strategy from lemongrass leaves hydrolysates using Phanerochaete chrysosporium ATCC 24725 in batch culture

Galadima, Ahmed Ibrahim and Salleh, Madihah Md and Hussin, Huszalina and Shiong, Chong Chun and Yahaya, Adibah and Mohamad, Shaza Eva and Aziz, Suraini Abdu and Yusof, Nor Nadiah Mohamad and Al-Junid, Amir Feisal Merican (2022) Improvement of biovanillin production with two-stage pH control strategy from lemongrass leaves hydrolysates using Phanerochaete chrysosporium ATCC 24725 in batch culture. Biomass Conversion and Biorefinery, 12 (7). pp. 2727-2736. ISSN 2190-6815, DOI https://doi.org/10.1007/s13399-020-00980-y.

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Abstract

The biovanillin production was influenced by varying the culture pH via single control strategy conducted by separate experiments during the fermentation processes. Highest biovanillin production (124 mg/L) with 32% molar yield at culture pH 6.0 from the one-stage control method was observed. Specific growth rates (mu) of thePhanerochaete chrysosporiumand biovanillin production decreased by decreasing the culture pH from 6.0 to 3.5, which indicated that lower culture pH was not adequately apposite for biovanillin production usingPhanerochaete chrysosporiumin a 2-L stirred tank bioreactor. The development of two-stage control strategies had improved the biovanillin production (131 mg/L) and cell concentration (13.0 g/L) by about 6 and 5%, respectively. Therefore, the most influential control strategy for higher biovanillin production was discovered not to control the culture pH of the fermentation during active growth phase of thePhanerochaete chrysosporium, while the production phase should be controlled at pH 6.0.

Item Type: Article
Funders: Ministry of Energy, Science, Technology, Environment and Climate Change (MESTECC), Malaysia (Grant No: R.J130000.7945.4s128)
Uncontrolled Keywords: Batch culture; Biovanillin; Lemongrass leaves hydrolysates; Phanerochaete chrysosporiumATCC 24725; pH control strategy; 2-liter stirred tank bioreactor
Subjects: Q Science > QH Natural history > QH301 Biology
Divisions: Faculty of Science > Institute of Biological Sciences
Depositing User: Ms. Juhaida Abd Rahim
Date Deposited: 20 Oct 2023 02:53
Last Modified: 20 Oct 2023 02:53
URI: http://eprints.um.edu.my/id/eprint/41841

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