Zen, Lee Phone Youth and Lai, Meng Yee and Rozlan, Syaza Izzati binti and Hamid, Mohd Hafizi Abdul and Jelip, Jenarun and Mudin, Rose Nani and Lau, Yee Ling (2022) End-point detection of loop-mediated isothermal amplification (LAMP) on malaria by direct observation with colorimetric dyes. Experimental Parasitology, 239. ISSN 0014-4894, DOI https://doi.org/10.1016/j.exppara.2022.108310.
Full text not available from this repository.Abstract
In order to ascertain the results of the LAMP technique, different end-point detection methods can be employed. However, these methods require sophisticated equipment. To simplify current end-point detection methods for the diagnosis of malaria, we propose the incorporation of colorimetric dyes: malachite green (MG), phenol red (PR), and xylenol orange (XO) in the LAMP assay. To evaluate the optimum concentration of dyes, 5 different concentrations (50 mu M, 75 mu M, 100 mu M, 125 mu M, and 150 mu M) were used with buffer pH 8.5 and pH 8.8, respectively. The results showed that 125 mu M of MG at pH 8.8 produced the most obvious colour change. A total of 71 clinical blood samples of Plasmodium knowlesi, Plasmodium malariae, Plasmodium vivax, Plasmodium falciparum, and healthy donors were tested using MG -LAMP. It showed 100% sensitivity and specificity. The simplicity and affordability of this method make it ideal to be used as an end-point detection method for malaria diagnosis in resource limited settings.
| Item Type: | Article |
|---|---|
| Funders: | Ministry of Education, Malaysia (Grant No: LR002D-2018 & LRGS/1/2018/UM/01/1/4) |
| Uncontrolled Keywords: | Rapid detection; Liquid-chromatography; Leucomalachite green; Malachite green; Assay |
| Subjects: | R Medicine > R Medicine (General) |
| Divisions: | Faculty of Medicine > Parasitology Deparment |
| Depositing User: | Ms. Juhaida Abd Rahim |
| Date Deposited: | 27 Oct 2023 03:43 |
| Last Modified: | 27 Oct 2023 03:43 |
| URI: | http://eprints.um.edu.my/id/eprint/41684 |
Actions (login required)
 |
View Item |
