Liu, Yi and Xiao, Ai-Ping and Cheng, Hao and Liu, Liang-Liang and Kong, Kin Weng and Liu, Hong-Yan and Wu, Ding-Tao and Li, Hua-Bin and Gan, Ren-You (2022) Phytochemical differences of hemp (Cannabis sativa L.) leaves from different germplasms and their regulatory effects on lipopolysaccharide-induced inflammation in Matin-Darby canine kidney cell lines. Frontiers in Nutrition, 9. ISSN 2296-861X, DOI https://doi.org/10.3389/fnut.2022.902625.
Full text not available from this repository.Abstract
The increasing demand of hemp (Cannabis sativa L.) has attracted more interest in exploring its phytochemical profile and bioactivities, such as anti-inflammatory effect. In this study, the phytochemicals of different hemp leaves were investigated, with the content order: total saponins content (TSC) > total alkaloids content (TAC) > total phenolics content (TPC) > total flavonoids content (TFC) > cannabinoids. Hemp leaves from Shanxi accumulated higher flavonoids and cannabinoids (i.e., THC, CBD, and CBN), while phenolics were more abundant in those from Hunan. A lipopolysaccharide (LPS)-induced inflammatory Matin-Darby canine kidney (MDCK) cell model was established to evaluate the anti-inflammatory effects of hemp leaf extracts. Hemp leaf extracts, especially the D129 and c7, significantly increased cell viability of LPS-induced inflammatory MDCK cells, and D132 significantly decreased the secretion of pro-inflammatory cytokines (TNF-alpha and IL-6) and the lactate dehydrogenase (LDH) activity. Except for c12, other hemp leaf extracts obviously decreased the cell morphological damage of LPS-induced inflammatory MDCK cells. The correlation analysis revealed that cannabinol (CBN) and TPC showed the strongest correlation with anti-inflammatory activities, and hierarchical clustering analysis also showed that hemp germplasms from Shanxi might be good alternatives to the common cultivar Ym7 due to their better anti-inflammatory activities. These results indicated that hemp leaves were effective in LPS-induced inflammatory MDCK cells, and flavonoids and cannabinoids were potential geographical markers for distinguishing them, which can provide new insights into the anti-inflammatory effect of hemp leaves and facilitate the application of hemp leaves as functional ingredients against inflammatory-related disorders.
| Item Type: | Article |
|---|---|
| Funders: | Local Financial Funds of National Agricultural Science and Technology Center, Chengdu (Grant No: NASC2021KR08 & NASC2021TD01) |
| Uncontrolled Keywords: | Hemp germplasm; Hemp leaves; Phytochemicals; Anti-inflammatory effects; Matin-Darby canine kidney cells |
| Subjects: | Q Science > QH Natural history > QH301 Biology R Medicine > R Medicine (General) |
| Divisions: | Faculty of Medicine |
| Depositing User: | Ms. Juhaida Abd Rahim |
| Date Deposited: | 27 Nov 2023 02:02 |
| Last Modified: | 27 Nov 2023 02:02 |
| URI: | http://eprints.um.edu.my/id/eprint/41599 |
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