Setiawan, Agus Budi and Purwantoro, Aziz and Teo, Chee How and Nhi, Phan Thi Phuong and Kato, Kenji and Kikuchi, Shinji and Koba, Takato (2022) The divergence of chromosome structures and 45S ribosomal DNA organization in Cucumis debilis inferred by comparative molecular cytogenetic mapping. Plants-Basel, 11 (15). ISSN 2223-7747, DOI https://doi.org/10.3390/plants11151960.
Full text not available from this repository.Abstract
Cucumis debilis W.J.de Wilde & Duyfjes is an annual and monoecious plant. This species is endemic to Southeast Asia, particularly Vietnam. However, C. debilis is rarely studied, and no detailed information is available regarding its basic chromosome number, 45S ribosomal DNA (rDNA) status, and divergence among other Cucumis species. In this study, we characterized the morphological characters and determined and investigated the basic chromosome number and chromosomal distribution of 45S rDNA of C. debilis using the fluorescent in situ hybridization (FISH) technique. A maximum likelihood tree was constructed by combining the chloroplast and internal transcribed spacer of 45S rDNAs to infer its relationship within Cucumis. C. debilis had an oval fruit shape, green fruit peel, and protrusion-like white spots during the immature fruit stage. FISH analysis using 45S rDNA probe showed three pairs of 45S rDNA loci located at the terminal region in C. debilis, similar to C. hystrix. Meanwhile, two, two, and five pairs of 45S rDNA loci were observed for C. melo, C. metuliferus, and C. sativus, respectively. One melon (P90) and cucumber accessions exhibited different chromosomal localizations compared with other members of Cucumis. The majority of Cucumis species showed the terminal location of 45S rDNA, but melon P90 and cucumber exhibited terminal-interstitial and all interstitial orientations of 45S rDNA loci. Based on molecular cytogenetics and phylogenetic evidence, C. debilis is more closely related to cucumber than melon. Therefore, C. debilis may serve as a potential parental accession for genetic improvement of cucumber through interspecific hybridization.
| Item Type: | Article |
|---|---|
| Funders: | None |
| Uncontrolled Keywords: | C. debilis fruit; C. debilis rDNA loci; Cucumis chromosomes; divergence of rDNA loci |
| Subjects: | S Agriculture > S Agriculture (General) |
| Divisions: | Deputy Vice Chancellor (Research & Innovation) Office > Centre for Research in Biotechnology for Agriculture |
| Depositing User: | Ms. Juhaida Abd Rahim |
| Date Deposited: | 08 Nov 2023 08:22 |
| Last Modified: | 08 Nov 2023 08:22 |
| URI: | http://eprints.um.edu.my/id/eprint/41574 |
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