Alwakil, Nurul Huda and Mohamad Annuar, Mohamad Suffian and Jalil, Mahanom (2022) Synergistic effects of plant growth regulators and elicitors on alpha-humulene and zerumbone production in Zingiber zerumbet smith adventitious root cultures. Molecules, 27 (15). ISSN 1420-3049, DOI https://doi.org/10.3390/molecules27154744.
Full text not available from this repository.Abstract
Zingiber zerumbet, also known as `Lempoyang', possesses various phytomedicinal properties, such as anticancer, antimicrobial, anti-inflammatory, antiulcer, and antioxidant properties. Secondary metabolites possessing such properties i.e., zerumbone and alpha-humulene, are found dominantly in the plant rhizome. Synergistic effects of plant growth hormones and elicitors on in vitro alpha-humulene and zerumbone production, and biomass growth, in adventitious root culture (AdRC) of Z. zerumbet cultivated in a two-stage culture are reported. The culture was induced by supplementation of 1.0 mg/L NAA and 2.0 mg/L IBA (dark), and subsequently maintained in medium supplemented with 1 mg/L NAA and 3 mg/L BAP (16:08 light-dark cycle), yielded the production of zerumbone at 3440 +/- 168 mu g/g and alpha-humulene at 3759 +/- 798 mu g/g. Synergistic elicitation by 400 mu M methyl jasmonate (MeJa) and 400 mu M salicylic acid (SA) resulted in a 13-fold increase in zerumbone (43,000 +/- 200 mu g/g), while 400 mu M MeJa and 600 mu M SA produced a 4.3-fold increase in alpha-humulene (15,800 +/- 5100 mu g/g) compared to control.
| Item Type: | Article |
|---|---|
| Funders: | Centre for Research in Biotechnology for Agriculture (CEBAR), Universiti Malaya (Grant No: RU006-2017), Ministry of Education, Malaysia (Grant No: MRUN_2019) |
| Uncontrolled Keywords: | Phytomedicinal; Secondary metabolites; In vitro; Methyl jasmonate; Salicylic acid |
| Subjects: | Q Science > QD Chemistry Q Science > QH Natural history > QH301 Biology S Agriculture > S Agriculture (General) |
| Divisions: | Centre for Foundation Studies in Science > Biology Division Faculty of Science > Institute of Biological Sciences Deputy Vice Chancellor (Research & Innovation) Office > Centre for Research in Biotechnology for Agriculture |
| Depositing User: | Ms. Juhaida Abd Rahim |
| Date Deposited: | 10 Nov 2023 01:26 |
| Last Modified: | 10 Nov 2023 01:26 |
| URI: | http://eprints.um.edu.my/id/eprint/41550 |
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