Lai, Meng Yee and Bukhari, Fatma Diyana Mohd and Zulkefli, Nur Zulaikha and Ismail, Ilyiana and Mustapa, Nur Izati and Soh, Tuan Suhaila Tuan and Hassan, Afifah Haji and Peariasamy, Kalaiarasu M. and Lee, Yee Leng and Suppiah, Jeyanthi and Thayan, Ravindran and Isa, Mohd Khairi Mat and Wahid, Nur Zafirah Abdul and Lau, Yee Ling (2022) Clinical testing on SARS-CoV-2 swab samples using reverse-transcription loop-mediated isothermal amplification (RT-LAMP). BMC Infectious Diseases, 22 (1). ISSN 1471-2334, DOI https://doi.org/10.1186/s12879-022-07684-w.
Full text not available from this repository.Abstract
Background High cost of commercial RNA extraction kits limits the testing efficiency of SARS-CoV-2. Here, we developed a simple nucleic acid extraction method for the detection of SARS-CoV-2 directly from nasopharyngeal swab samples. Methods A pH sensitive dye was used as the end point detection method. The obvious colour changes between positive and negative reactions eliminates the need of other equipment. Results Clinical testing using 260 samples showed 92.7% sensitivity (95% CI 87.3-96.3%) and 93.6% specificity (95% CI 87.3-97.4%) of RT-LAMP. Conclusions The simple RNA extraction method minimizes the need for any extensive laboratory set-up. We suggest combining this simple nucleic acid extraction method and RT-LAMP technology as the point-of care diagnostic tool.
| Item Type: | Article |
|---|---|
| Funders: | Prototype Research Grant Scheme (PRGS) from the Ministry of Education, Malaysia (Grant No: PR0012020B & PRGS/2/2020/SKK09/UM/02/1) |
| Uncontrolled Keywords: | Diagnosis; RT-LAMP; SARS-CoV-2; Phenol red; pH sensitive indicator |
| Subjects: | R Medicine > R Medicine (General) |
| Divisions: | Faculty of Medicine > Parasitology Deparment |
| Depositing User: | Ms. Juhaida Abd Rahim |
| Date Deposited: | 03 Nov 2023 07:30 |
| Last Modified: | 03 Nov 2023 07:30 |
| URI: | http://eprints.um.edu.my/id/eprint/41532 |
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