Dubern, Jean-Frederic and Romero, Manuel and Mai-Prochnow, Anne and Messina, Marco and Trampari, Eleftheria and Naghra-van Gijzel, Hardeep and Chan, Kok-Gan and Carabelli, Alessandro M. and Barraud, Nicolas and Lazenby, James and Chen, Ye and Robertson, Shaun and Malone, Jacob G. and Williams, Paul and Heeb, Stephan and Camara, Miguel (2022) ToxR is a c-di-GMP binding protein that modulates surface-associated behaviour in Pseudomonas aeruginosa. npj Biofilms and Microbiomes, 8 (1). ISSN 2055-5008, DOI https://doi.org/10.1038/s41522-022-00325-9.
Full text not available from this repository.Abstract
Pseudomonas aeruginosa uses multiple protein regulators that work in tandem to control the production of a wide range of virulence factors and facilitate rapid adaptation to diverse environmental conditions. In this opportunistic pathogen, ToxR was known to positively regulate the production of the major virulence factor exotoxin A and now, through analysis of genetic changes between two sublines of P. aeruginosa PAO1 and functional complementation of swarming, we have identified a previously unknown role of ToxR in surface-associated motility in P. aeruginosa. Further analysis revealed that ToxR had an impact on swarming motility by regulating the Rhl quorum sensing system and subsequent production of rhamnolipid surfactants. Additionally, ToxR was found to tightly bind cyclic diguanylate (c-di-GMP) and negatively affect traits controlled by this second messenger including reducing biofilm formation and the expression of Psl and Pel exopolysaccharides, necessary for attachment and sessile communities matrix scaffolding, in P. aeruginosa. Moreover, a link between the post-transcriptional regulator RsmA and toxR expression via the alternative sigma factor PvdS, induced under iron-limiting conditions, is established. This study reveals the importance of ToxR in a sophisticated regulation of free-living and biofilm-associated lifestyles, appropriate for establishing acute or chronic P. aeruginosa infections.
| Item Type: | Article |
|---|---|
| Funders: | UK Research & Innovation (UKRI) Innovate UK, Hartree Centre, BB/R012415/1, H50001-A-000027, ISP grant, BB/J004553/1 |
| Uncontrolled Keywords: | Pseudomonas aeruginosa; ToxR; Exotoxin-A expression |
| Subjects: | Q Science > QR Microbiology |
| Divisions: | Faculty of Science > Institute of Biological Sciences |
| Depositing User: | Ms. Juhaida Abd Rahim |
| Date Deposited: | 23 Nov 2023 09:18 |
| Last Modified: | 23 Nov 2023 09:18 |
| URI: | http://eprints.um.edu.my/id/eprint/41531 |
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