Mitochondrial DNA and nuclear DNA indicate that the Japanese Fasciola species is F-gigantica

Hashimoto, K.; Watanobe, T.; Liu, C.X.; Init, I.; Blair, D.; Ohnishi, S.; Agatsuma, T. (1997) Mitochondrial DNA and nuclear DNA indicate that the Japanese Fasciola species is F-gigantica. Parasitology Research, 83 (3). pp. 220-225. ISSN 0044-3255

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    Abstract

    For elucidation of the taxonomic status of the Japanese Fasciola species, whole mitochondrial DNA of Fasciola hepatica from Australia, F. gigantica from Malaysia, and Fasciola sp. from Japan was digested with three four-base-cutting endonucleases: HinfI, MspI, and RsaI. The resulting digestion patterns showed that for each enzyme there were some bands specific for each geographical isolate and that the Japanese Fasciola sp, shared more bands with F. gigantica than with F. hepatica. Nucleotide sequences of two regions, the second internal transcribed spacer (ITS2) of the nuclear ribosomal RNA cluster and mitochondrial cytochrome c oxidase subunit I (COI), were also compared among them. The ITS2 sequence was highly conserved among the three isolates. F. gigantica and the Japanese Fasciola sp. were identical, but they differed from the Australian F. hepatica at six sites, one of which was a deletion. The COI sequence was less conserved but implied a similar relationship between the isolates. There seems no reason to regard the Japanese Fasciola sp. as anything other than a strain of F. gigantica.

    Item Type: Article
    Creators:
    1. Hashimoto, K.
    2. Watanobe, T.
    3. Liu, C.X.
    4. Init, I.(Department of Parasitology, Faculty of Medicine Building, University of Malaya, 50603 Kuala Lumpur, MALAYSIA)
    5. Blair, D.
    6. Ohnishi, S.
    7. Agatsuma, T.
    Journal or Publication Title: Parasitology Research
    Additional Information: Hashimoto, K Watanobe, T Liu, CX Init, I Blair, D Ohnishi, S Agatsuma, T
    Uncontrolled Keywords: Digenea
    Subjects: R Medicine
    Divisions: Faculty of Medicine
    Depositing User: Mr Jenal S
    Date Deposited: 12 Dec 2012 12:07
    Last Modified: 12 Dec 2012 12:07
    URI: http://eprints.um.edu.my/id/eprint/4128

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