Polymerase chain reaction identification of three members of the Anopheles sundaicus (Diptera: Culicidae) complex, malaria vectors in Southeast Asia

Dusfour, I.; Blondeau, J.; Harbach, R.E.; Vythilingham, I.; Baimai, V.; Trung, H.D.; Sochanta, T.; Bangs, M.J.; Manguin, S. (2007) Polymerase chain reaction identification of three members of the Anopheles sundaicus (Diptera: Culicidae) complex, malaria vectors in Southeast Asia. Journal of Medical Entomology, 44 (5). pp. 723-731. ISSN 0022-2585

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    Abstract

    Anopheles sundaicus s.l., a major malaria vector taxon, occurs primarily along coastal areas and on islands in Southeast Asia. Our previous studies using cytochrome oxidase I, cytochrome-b, and internal transcribed spacer 2 markers discriminated three allopatric species: An. sundaicus s.s. in northern Borneo, An. epiroticus in Southeast Asia, and An. sundaicus E on Sumatra and Java, Indonesia. Morphological comparisons of three developmental stages did not reveal unique diagnostic characters that could reliably distinguish the three species. Therefore, we developed a multiplex polymerase chain reaction (PCR) assay based on two mitochondrial DNA markers to unambiguously identify them. This PCR was tested on 374 specimens from 24 different geographical populations, expanding our knowledge of the distribution of these species.

    Item Type: Article
    Creators:
    1. Dusfour, I.
    2. Blondeau, J.
    3. Harbach, R.E.
    4. Vythilingham, I.(Department of Parasitology, Faculty of Medicine Building, University of Malaya, 50603 Kuala Lumpur, MALAYSIA)
    5. Baimai, V.
    6. Trung, H.D.
    7. Sochanta, T.
    8. Bangs, M.J.
    9. Manguin, S.
    Journal or Publication Title: Journal of Medical Entomology
    Additional Information: Department of Parasitology, Faculty of Medicine Building, University of Malaya, 50603 Kuala Lumpur, MALAYSIA
    Uncontrolled Keywords: Anopheles sundaicus; polymerase chain reaction; malaria vector
    Subjects: R Medicine
    Divisions: Faculty of Medicine
    Depositing User: Miss Nur Jannatul Adnin Ahmad Shafawi
    Date Deposited: 12 Dec 2012 11:58
    Last Modified: 11 Dec 2013 10:01
    URI: http://eprints.um.edu.my/id/eprint/4123

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