Polymerase chain reaction identification of three members of the Anopheles sundaicus (Diptera: Culicidae) complex, malaria vectors in Southeast Asia

Dusfour, I. and Blondeau, J. and Harbach, R.E. and Vythilingam, Indra and Baimai, V. and Trung, H.D. and Sochanta, T. and Bangs, M.J. and Manguin, S. (2007) Polymerase chain reaction identification of three members of the Anopheles sundaicus (Diptera: Culicidae) complex, malaria vectors in Southeast Asia. Journal of Medical Entomology, 44 (5). pp. 723-731. ISSN 0022-2585, DOI 17915501.

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Abstract

Anopheles sundaicus s.l., a major malaria vector taxon, occurs primarily along coastal areas and on islands in Southeast Asia. Our previous studies using cytochrome oxidase I, cytochrome-b, and internal transcribed spacer 2 markers discriminated three allopatric species: An. sundaicus s.s. in northern Borneo, An. epiroticus in Southeast Asia, and An. sundaicus E on Sumatra and Java, Indonesia. Morphological comparisons of three developmental stages did not reveal unique diagnostic characters that could reliably distinguish the three species. Therefore, we developed a multiplex polymerase chain reaction (PCR) assay based on two mitochondrial DNA markers to unambiguously identify them. This PCR was tested on 374 specimens from 24 different geographical populations, expanding our knowledge of the distribution of these species.

Item Type:	Article
Funders:	UNSPECIFIED
Additional Information:	Department of Parasitology, Faculty of Medicine Building, University of Malaya, 50603 Kuala Lumpur, MALAYSIA
Uncontrolled Keywords:	Anopheles sundaicus; polymerase chain reaction; malaria vector
Subjects:	R Medicine
Divisions:	Faculty of Medicine
Depositing User:	Miss Nur Jannatul Adnin Ahmad Shafawi
Date Deposited:	12 Dec 2012 03:58
Last Modified:	08 Jan 2020 08:43
URI:	http://eprints.um.edu.my/id/eprint/4123

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